Addgene

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Showing: 4521 - 4540 of 54675 results

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pENTR1a-mCherry-STIM1

Plasmid

#114176

Purpose

Gateway entry clone containing mCherry-STIM1

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

STIM1 (STIM1 Human)

Use

Gateway entry clone

Tags

mCherry (inserted after signal peptide)

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Parkin-IRES-zeo

Plasmid

#61727

Purpose

Expression of human Parkin fused to mCherry

Depositor

Douglas Green, Stephen Tait

Article

Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

Insert

Parkin (PRKN Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CNOT2

Plasmid

#155466

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CNOT2 (CNOT2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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P-rTetO-EGFP-T2A-RPL22-3xHA

Plasmid

#170326

Purpose

Tetracycline-inducible expression of HA and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-proα2(I)-G610C

Plasmid

#119827

Purpose

Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

Depositor

Sergey Leikin

Article

Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

Insert

Type I procollagen α2 chain (Col1a2 Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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DCP1A

Plasmid

#155498

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DCP1A (DCP1A Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mIRFP670nuc-TEV-Xph20-ETWV

Plasmid

#133044

Purpose

Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20-ETWV

Use

Tags

NLS, TEV protease + TEV cleavage site, and miRFP6…

Expression

Mammalian

Mutation

S63K

Promoter

CAG

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAG87 FAST

Plasmid

#130719

Purpose

Expresses His-tagged FAST (also called YFAST) in bacteria

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

FAST

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ADD1

Plasmid

#155397

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADD1 (ADD1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-EGFP-T2A-Puro

Plasmid

#170318

Purpose

Expresses FLAG and EGFP tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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DDX5

Plasmid

#155523

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX5 (DDX5 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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TERT

Plasmid

#156078

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TERT (TERT Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL WT

Plasmid

#107159

Purpose

For mammalian expression of Flag-hPKL WT

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL WT (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAG109 H2B-FAST

Plasmid

#130722

Purpose

Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

H2B-FAST

Use

Tags

myc-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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P-rTetO-mCherry-T2A-RPL22-1xV5

Plasmid

#170325

Purpose

Tetracycline-inducible expression of V5 and mCherry tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XFlag-P2A-mCherry-T2A-Puro

Plasmid

#170322

Purpose

Expresses FLAG and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1-EXO1b-D173A

Plasmid

#111622

Purpose

Expression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

Insert

Exonuclease 1b D173A (EXO1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-mGSDME F2A

Plasmid

#154878

Purpose

Expresses mGSDME F2A in mammalian cells

Depositor

Judy Lieberman

Article

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (Gsdme Mouse)

Use

Lentiviral

Tags

Expression

Mutation

changes Phenylalanine 2 to Alanine

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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