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Plasmid#137086PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherryDepositorInsertmCherry
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP3041
Plasmid#137083PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with GFP11-3xFlag-tagRFPDepositorInsertGFP11::3xFlag::tagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRB20
Plasmid#52559Purposeserves as PCR-template to generate HR donors for C-terminal YFP-tagDepositorInserteYFP and Blasticigin resistance cassette
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-BtH
Plasmid#133392PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP1680
Plasmid#137088PurposePlasmid for PCR-generated donor DNA for rapid tagging of proteins with tagRFPDepositorInserttagRFP
UsePcr template for donor dnaTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTGC-U6.2
Plasmid#112809PurposePCR template vector for amplifying gRNAcore-pU6.3 promoter fragmentDepositorInsertU6:2-gRNAcore
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Kan
Plasmid#232102PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan
Plasmid#232099PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg
Plasmid#232098PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDW108
Plasmid#227194PurposeTemplate for multi-nucleosome arrayDepositorInsert64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-167:CDH5-mEGFP
Plasmid#217425PurposeHomology arms and mEGFP sequence for C-terminus tagging of human cadherin 5DepositorInsertCDH5 Homology Arms with mEGFP (CDH5 Human)
UseCRISPR; Donor templateTagsmEGFPExpressionMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCmini-UGCG-EGFP
Plasmid#225878PurposePlasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertionDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL6000
Plasmid#216797PurposePlatform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinaseDepositorTypeEmpty backboneUseCre/Lox; Leishmania dicre inducible expressionTags6xHAExpressionMutationPromoterrRNA promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1397-DddtoxA-N
Plasmid#191596PurposeptpTALECD vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1397-DddtoxA-N
Plasmid#191597PurposeptpTALECD vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1397-DddtoxA-N
Plasmid#191598PurposeptpTALECD vector assembly, Entry clone 3 with attL2 and L3DepositorInsertC and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1397-DddtoxA-C
Plasmid#191583PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1397-DddtoxA-C
Plasmid#191584PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC and N termini of platinum TALEN, 1/2 a cytidine deaminase domain of DddA of B. cenocepacia, and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only