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Plasmid#145753PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHcPro (GB0067)
Plasmid#68196PurposeProvides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid partDepositorInsertTEV HcPro coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046908
Plasmid#145603PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046878
Plasmid#145743PurposeBacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonucleaseDepositorInsertSARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046904
Plasmid#145591PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG584
Plasmid#40191DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoPL
Plasmid#114467PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoPLDepositorInsertBoPL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx TOM20 mVenus BoLL
Plasmid#114465PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLLDepositorInsertBoLL
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEX Tom20 mVenus BoLD
Plasmid#114468PurposeMammalian expression of mitochondrially targeted, mVenus labelled BoLDDepositorInsertBoLD
UseTagsHIS, TEV and FLAG and TOM20 and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(81-437)
Plasmid#87734PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-80PromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG587
Plasmid#40196DepositorInsertMex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS458A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234A_S235A
Plasmid#40634DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionMammalianMutationT234A_S235APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234E_S235E
Plasmid#40635DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, and Thrombin CleavageExpressionBacterialMutationT234E_S235EPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234E
Plasmid#40636DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, and Thrombin CleavageExpressionBacterialMutationT234EPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_T234A
Plasmid#40637DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV cleavage, and Thrombin cleavageExpressionBacterialMutationT234APromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1_OCT4_S235E
Plasmid#40638DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsGST, TEV Cleavage, and Thrombin CleavageExpressionBacterialMutationS235EPromotertacAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG585
Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only