Addgene

We narrowed to 6,564 results for: ski

Showing: 4481 - 4500 of 6564 results

Results per page:

Sort results by:

HiBiT-FZD4

Plasmid

#195842

Purpose

Expresses human FZD4 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD4 (FZD4 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

Plasmid

#192184

Purpose

Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

Plasmid

#192183

Purpose

Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
His-FLAG-Tev_ACTB (beta-actin)

Plasmid

#188453

Purpose

Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

ACTB (ACTB Human)

Use

Tags

6xHis-FLAG

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-FZD6

Plasmid

#195844

Purpose

Expresses human FZD6 with an N-terminal HiBiT; contains M345L

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD6 (FZD6 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

M345L

Promoter

CMV

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-MYO7A-TAIL

Plasmid

#89585

Purpose

Expression plasmid for a positive control bait protein in NanoSPD 2.0 assays.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO7A (Myo7a Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Truncation encoding amino acids 870-2166 of MYO7A…

Promoter

CMV

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-TRC.mKO2_shGFP

Plasmid

#110318

Purpose

Control (Target CAAGCTGACCCTGAAGTTCAT), silence GFP and express monomeric Kusabira-Orange2

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GFP

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PL-5LTR-GW-A

Plasmid

#138480

Purpose

For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG.SF-iGluSnFR.A184S

Plasmid

#106198

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184S

Use

AAV

Tags

Expression

Mutation

GltI: A184S

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HiBiT-FZD2

Plasmid

#195840

Purpose

Expresses human FZD2 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD2 (FZD2 Human)

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat3-SH2

Plasmid

#46513

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat3 (STAT3 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 584-700

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX FynA-SH2

Plasmid

#46429

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

FynA (FYN Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 142-251

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA6-N-Flag-SREBP1

Plasmid

#134283

Purpose

Expresses Flag-tagged SREBP1 in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SREBP1 (Srebf1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

mouse SREBP1 isoform a precursor

Promoter

CMV

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Expression

Mutation

Promoter

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pSH-EF1a-OsTIR1.F74A-mCherry-IRES-puro

Plasmid

#216247

Purpose

Express OsTIR1.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

OsTIR1.F74A

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-TetO-Tead4

Plasmid

#128832

Purpose

dox-dependent expression of mouse Tead4

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

Insert

Tead4 (Tead4 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.SF-iGluSnFR.A184S

Plasmid

#106192

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1

Insert

SF-iGluSnFR.A184S

Use

AAV

Tags

Expression

Mutation

GltI: A184S

Promoter

GFAP

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CLXSN 5myc-CDC6-mut

Plasmid

#109333

Purpose

express tagged Cdc6 mutant

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

CDC6 (CDC6 Human)

Use

Retroviral

Tags

5x Myc

Expression

Mammalian

Mutation

R56A, L59A, K81A, E82A, N83A

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge