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Plasmid#180023PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.DepositorInsertGB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)-Strep
Plasmid#164444PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G)-Strep.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G)-Strep (PDCD6IP Human)
UseTagsGB1-His6-TEV and Strep tag (WSHPQFEK)ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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p3R112C/T180CBM3
Plasmid#85090PurposeExpresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coliDepositorInsertThe R112C/T180C variant of PCNA3 fused to P450 BM3
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3PromoterT7 promoterAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
RC(14-3-3ε)-LgBit
Plasmid#208926PurposeRecognition domain for in vitro RSK-activity sensorDepositorInsert14-3-3ε (YWHAQ Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VA
Plasmid#186454PurposepTarget containing protospacer for FnCas12a targetingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_priming_VA
Plasmid#186455PurposepTarget containing protospacer with a mismatch on position 1, for FnCas12a targetingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTarget_PS_VB
Plasmid#186456PurposepTarget containing protospacer for AaCas12b targetingDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEUK028
Plasmid#180826PurposeT7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coliDepositorArticleInsertSwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
UseTags6X HisExpressionBacterialMutationPromoterT7Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEUK037
Plasmid#180829PurposeT7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coliDepositorArticleInsertCnGdmB (CNE_RS35790 Cupriavidus necator N-1)
UseTags6X HisExpressionBacterialMutationPromoterT7Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
RGD-I27-CC
Plasmid#85393PurposeMTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertRGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CFa
Plasmid#85089PurposeExpresses PCNA1 variant (G108C) fused to an FAD domain of P450 BM3 (A74G/C773S/C810S) in E. coliDepositorInsertThe G108C variant of PCNA1 fused to an FAD domain of P450 BM3
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1, A74G/C773S/C810S in FAD domain of…PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSaPIPLC His
Plasmid#173810PurposeExpression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertStaphylococcus aureus plc
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only