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Plasmid#186787PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasmDepositorInsertcytoplasmic NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
nuclear NAD+ biosensor
Plasmid#186789PurposeNAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleusDepositorInsertnuclear NAD+ biosensor
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mCherry-CAAX
Plasmid#129285PurposeLentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequenceDepositorInsertFluorescent reporter mCherry fused to CAAX polybasic sequence
UseLentiviralTagsCAAXExpressionMammalianMutationPromoterCMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPIDNB
Plasmid#169901PurposeEmpty dox inducible vector with piggyBac, insulators, and a DTSDepositorTypeEmpty backboneUseAivian expressionTagsExpressionMammalianMutationPromoterDox inducibleAvailable sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
ERM-APEX2
Plasmid#79055PurposeSoybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.DepositorInsertAPEX2 anchored to the cytosolic face of the ER membrane
UseLentiviralTagsResidues 1-27 of P450 oxidase 2C1 and V5ExpressionBacterial and MammalianMutationPromoterCMV immearly promotorAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-mCherry
Plasmid#20938PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-T2A-GFP
Plasmid#53190Purpose3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFPDepositorInserthumanized Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationPromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-WT mouse MyoD-T2A-dsRedExpress2
Plasmid#60624PurposeExpresses WT mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertWT mouse MyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO2
Plasmid#24317DepositorInserthuman AGO2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
plk1 T210A
Plasmid#132965PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 T210A (PLK1 Human)
UseTagsExpressionMammalianMutationT210APromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti TurboRFP BlastR
Plasmid#102343PurposeLentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neuronsDepositorInsertTurboRFP
UseLentiviralTagsN/AExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425 GAL Pong TPase L418A, L420A
Plasmid#145795PurposemPing Yeast Transposition AssayDepositorInsertPong TPase L418A, L420A
UseTagsExpressionYeastMutationL418A, L420APromoterAvailable sinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV hUbC-dCas9 VP64-T2A-GFP
Plasmid#53192PurposeCo-expresses human optimized S. pyogenes dCas9-VP64 and GFPDepositorInserthumanized dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNox4
Plasmid#69352Purposeexpresses human Nox4 in mammalian cellsDepositorInserthNOX4 (NOX4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVE-eGFP
Plasmid#52581PurposeLentiviral transfer vector driving expression of eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9-T2A-GFP
Plasmid#53191PurposeCo-expresses human optimized S. pyogenes dCas9 and GFPDepositorInserthumanized dead Cas9 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP
Plasmid#66707Purposeexpresses a single gRNA and VP64-dCas9-VP64DepositorInsertshU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhU6 and hUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only