Addgene

We narrowed to 18,525 results for: OMP;

Showing: 4481 - 4500 of 18525 results

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pET47b-T7-dAsCas12f

Plasmid

#204641

Purpose

Expression of an inactive AsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

inactive AsCas12f

Use

Tags

6xHis

Expression

Bacterial

Mutation

D225A

Promoter

T7

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag RbBP5

Plasmid

#15550

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

ITPKA

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBS human c-Myc

Plasmid

#14971

Depositor

Joan Massague

Article

Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

Insert

c-Myc (MYC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Article

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Use

Tags

His6 followed by a thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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SauriABEmax

Plasmid

#135968

Purpose

Expresses ABEmax and nSauriCas9

Depositor

Yongming Wang

Article

Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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rArc

Plasmid

#178206

Purpose

Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

Depositor

Stefan Heinemann

Article

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rArc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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myc-hULK2

Plasmid

#31966

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK2 (ULK2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSplit-ON-hDib1

Plasmid

#58787

Purpose

Express hDib1-LucN fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Use

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

Rab7

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Use

Tags

Expression

Mutation

Promoter

no promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPP444

Plasmid

#158802

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-3 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec31A

Plasmid

#66613

Purpose

YFP fusion of Sec31A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pWRS_1001

Plasmid

#192205

Purpose

lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

Depositor

William Sellers

Article

Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and RNAi

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA6

Plasmid

#19486

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 6 (HSPA6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

Plasmid

#171104

Purpose

Expresses sgRNA (ENO1 gRNA) in Candida auris.

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

sgRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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