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Plasmid#193627PurposeInducible expression of LuxI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LuxI is codon optimized for E. coli.DepositorInsertLuxI
UseTags6xHisExpressionBacterialMutationPromoterLux promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
UseTags6His and mycExpressionBacterialMutationPromoterpBADAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
RF4
Bacterial Strain#62072PurposeBL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.DepositorBacterial ResistanceNoneAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12C
Plasmid#81667PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12V
Plasmid#81665PurposeGateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12VPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_WT
Plasmid#81923PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12D
Plasmid#81651PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_WT
Plasmid#81751PurposeGateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.A59G
Plasmid#81662PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationA59GPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12A
Plasmid#81673PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12APromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.D33E
Plasmid#81659PurposeGateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationD33EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.G12S
Plasmid#81654PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationG12SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERBB2_p.V842I
Plasmid#81722PurposeGateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.DepositorInsertERBB2 (ERBB2 Human)
UseGateway entry vectorTagsExpressionMutationI655V; V842IPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_KRAS_p.E62K
Plasmid#81656PurposeGateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.DepositorInsertKRAS (KRAS Human)
UseGateway entry vectorTagsExpressionMutationE62KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ERBB2_p.R897Q
Plasmid#81714PurposeGateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.DepositorInsertERBB2 (ERBB2 Human)
UseGateway entry vectorTagsExpressionMutationI655V; R897QPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only