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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-TRE-dCas9-KRAB-MeCP2-BSD

Plasmid

#140690

Purpose

Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

Cas9m4-KRAB-MeCP2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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LZRS-Mek2-K101A (KA7)

Plasmid

#21191

Depositor

Paul Khavari

Insert

Mek2 (MAP2K2 Human)

Use

Retroviral

Tags

Expression

Mutation

K101A (catalytically inactive)

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
LZRS-Mek2-K101A (KA7)

Plasmid

#21191

Depositor

Paul Khavari

Insert

Mek2 (MAP2K2 Human)

Use

Retroviral

Tags

Expression

Mutation

K101A (catalytically inactive)

Promoter

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pMH-HA

Plasmid

#101767

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAF0687 Pa01_attP-mCherry donor

Plasmid

#193466

Purpose

Promoterless mcherry donor for Pa01

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA2

Plasmid

#26678

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 2 (KPNA2 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA2

Plasmid

#26678

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 . 11():63.

Insert

Karyopherin Alpha 2 (KPNA2 Human)

Use

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pScarlet-N1

Plasmid

#128060

Purpose

Mammalian expression vector for C terminal mScarlet-I fusions

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pScarlet-N1

Plasmid

#128060

Purpose

Mammalian expression vector for C terminal mScarlet-I fusions

Depositor

Oskar Laur

Type

Empty backbone

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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NLRP3-mNeonGreen

Plasmid

#200942

Purpose

NLRP3 fused with mNeonGreen to indicate its localization change

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET iGluSnFR3 v857

Plasmid

#175186

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

iGluSnFR3 v857

Use

Synthetic Biology

Tags

His tag, T7 leader, Xpress epitope

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6gRNA(lib)-PGKpuroT2ABFP library

Pooled Library

#104861

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only
ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.eGFP.WPRE.hGH

Plasmid

#105549

Purpose

AAV expression of EGFP from GFAP promoter

Depositor

James M. Wilson

Has Service

AAV5

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.eGFP.WPRE.hGH

Plasmid

#105549

Purpose

AAV expression of EGFP from GFAP promoter

Depositor

James M. Wilson

Has Service

AAV5

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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EGFR WT

Plasmid

#11011

Purpose

Retroviral construct for expressing human EGFR in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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