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Plasmid#134660PurposeCRISPR delivery and repair template delivery vectorDepositorInsertCRISPR guide RNA array
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2_RFP-60A
Plasmid#200394PurposePlasmid IVT template encoding RFP. NOTE: In the manuscript, "mCherry" was listed as the internal control for multiple fluorescence-based experiments, but it was actually RFP.DepositorInsertRFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_ABEmax_IVT
Plasmid#171761PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymeraseDepositorInsertABEmax
UseVector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLC-246
Plasmid#73190PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-tse2
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPD1028 ZF43x6-Compact(SV40Min)_EYFP
Plasmid#138912PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoterDepositorInsertZF43x6-Compact(SV40min)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Compact(SV40min)Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE4_IVT
Plasmid#171762PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE4
UseVector for in-vitro transcriptionTagsExpressionMutationPromoterT3 promoterAvailable sinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD1029 ZF43x6-Compact(CMVMin)_EYFP
Plasmid#138913PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a CMV minimal promoterDepositorInsertZF43x6-Compact(CMVmin)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Compact(CMVmin)Available sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayTagsExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fragment 1 Null
Plasmid#198729PurposeGateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.DepositorInsertgRNA expression cassette in pENTR4
UseGateway-compatible entry vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-3XHA
Plasmid#127881PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA taggingDepositorInsertGFP-Puro-3XHA (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5xUASG-iRFP-B3-timestamp-A
Plasmid#159925Purposetimestamp Template A inserted into the 3’ UTR of iRFP, in a second generation lentiviral backbone with the Vivid promoter, with RNA barcode CTG. AKA 149B3.DepositorInsertiRFP-Timestamp A
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLC-237
Plasmid#73191PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.DepositorInsertParaB-relE
UseTagsExpressionBacterialMutationPromoterParaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-PtH2B
Plasmid#133390PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-NQO1
Plasmid#136766PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Nqo1DepositorInsertNqo1 (Nqo1 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insert, V184A- Please see depositor co…PromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1
Plasmid#175360PurposeZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 PlatformDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-144:CTCF-mEGFP
Plasmid#168799PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCFDepositorInsertCTCF (CTCF Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pN-PITCh-FAID
Plasmid#127885PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID taggingDepositorInsertGFP-Puro-H3F-AID (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJW1354
Plasmid#69490PurposeGly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology armsDepositorInsertGSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
UseCRISPR; Template to generate cassettes for -media…TagsExpressionMutationPromoterAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only