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Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-GFP
Plasmid#132776PurposePrime editing in mammalian cells with co-translational GFP expressionDepositorInsertPE2-P2A-GFP
UseTagsSV40 NLSExpressionMammalianMutationSee manuscriptPromoterCMVAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 0.6kb promoter
Plasmid#20126DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 0.6kb promoter
Plasmid#20126DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
FCK-ChrimsonR-GFP
Plasmid#59049PurposeLentiviral expression of ChrimsonR under the CaMKII promoterDepositorInsertChrimsonR-GFP
UseLentiviralTagsGFPExpressionMammalianMutationK176RPromoterCaMKIIAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
FCK-ChrimsonR-GFP
Plasmid#59049PurposeLentiviral expression of ChrimsonR under the CaMKII promoterDepositorInsertChrimsonR-GFP
UseLentiviralTagsGFPExpressionMammalianMutationK176RPromoterCaMKIIAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-MKLP1
Plasmid#70145Purposemammalian expression of MKLP1 with an eGFP fusionDepositorInsertMKLP1 (KIF23 Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro
Plasmid#171991PurposeDelivers all prime editing nickase components in a single, puromycin selectable plasmidDepositorInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
UseTagsExpressionMammalianMutationPromoterCMV for Cas9, U6 for gRNAsAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFB1
Plasmid#116799PurposeLentiviral expression of TGFB1DepositorInsertTGFB1 (TGFB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFB1
Plasmid#116799PurposeLentiviral expression of TGFB1DepositorInsertTGFB1 (TGFB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT3T4
Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT3T4
Plasmid#50592PurposeCRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sitesDepositorInsertT3, gRNA scaffold, U6-1tplus, U6-26p, T4
UseCRISPR; Pcr templateTagsExpressionPlantMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LZRS-Mek2-K101A (KA7)
Plasmid#21191DepositorInsertMek2 (MAP2K2 Human)
UseRetroviralTagsExpressionMutationK101A (catalytically inactive)PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
LZRS-Mek2-K101A (KA7)
Plasmid#21191DepositorInsertMek2 (MAP2K2 Human)
UseRetroviralTagsExpressionMutationK101A (catalytically inactive)PromoterAvailable sinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only