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Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1244
Plasmid#155138PurposeExpresses MlaFED operon in E. coliDepositorInsertmlaFE-[6xHis-2xQH-TEV-mlaD]-mlaC operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1245
Plasmid#155139PurposeExpresses MlaF in E. coliDepositorInsert6xHis-TEV-mlaF
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1246
Plasmid#155141PurposeExpresses MlaB in E. coliDepositorInsert6xHis-TEV-mlaB
UseTagsExpressionMutationPromoterT7 promoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-MMP
Plasmid#112632PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcherDepositorInsertTriCatcher-MMP
UseTagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral matrix metalloproteinase-cleavable sequen…PromoterT5Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698N
Plasmid#92242PurposeExpress H698N mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698N mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AsparaginePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663K
Plasmid#92239PurposeExpress R663K mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663K mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to LysinePromoterT7 promoterAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-W695del
Plasmid#92241PurposeExpress W695-deletion mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-W695deletion mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationTryptophan 695 deletedPromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663Q
Plasmid#92240PurposeExpress R663Q mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663Q mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to GlutaminePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-H698A
Plasmid#92237PurposeExpress H698A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-H698A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationHistidine 698 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJExpress414_GmPOPB-R663A
Plasmid#92238PurposeExpress R663A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-R663A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationArginine 663 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
JARID1BA-c539
Plasmid#79643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertJARID1B (KDM5B Human)
UseBaculovirus expressionTags6x His and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
TbSIR2RP1
Plasmid#67242PurposeDetermine its activity and specificityDepositorInsertTbSIR2RP1
UseTagsMBP, TEV, Thrombin, HIS, and noneExpressionBacterialMutationPromoterp-lacAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-AlleyCat
Plasmid#41715DepositorInsertmutated C-terminal portion of calmodulin gene (M76-K148)
UseTagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationF92EPromoterT7Available sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.BSU37660
Plasmid#11535DepositorInsertphosphotransacetylase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
KRAS-A146T
Plasmid#154426PurposeA146T Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.DepositorInsertKRAS (KRAS Human)
UseTags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationPromoterT5Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
KTD101(DE3)
Bacterial Strain#138651PurposeKTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoterDepositorBacterial ResistanceNoneSpeciesEscherichia coliAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …PromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only