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Plasmid#165423PurposeGoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassetteInsertOs Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
UseSynthetic Biology; L1 p1(r) golden gate (moclo) h…TagsExpressionMutationPromoterOs Actin promoter :: Hygromycin resistance gene (…Available sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_deltaOB
Plasmid#166408Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsmycExpressionMammalianMutationΔOBPromoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQmod3C-GG
Plasmid#191350PurposeClostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IZ-ANXA6-mRuby3
Plasmid#184892PurposeStable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cellsDepositorInsertANXA6 (ANXA6 Human)
UseTagsmRuby3ExpressionMammalianMutationR587KPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQmod2C-GG
Plasmid#191347PurposeClostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUAL_2_araBAD
Plasmid#155176Purposesee pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom siteDepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgGAL4
Plasmid#121514PurposesgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgGAL4
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
LBR-mCherry
Plasmid#172446PurposeExpression of nuclear envelope protein LBR with a C-terminal mCherry tagDepositorInsertlamin B receptor (LBR Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET21a - Ec-ClpX
Plasmid#111516PurposeExpresses E. coli ClpX with a C-terminal His tagDepositorInsertClpX
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE
Plasmid#89608PurposeExpression of Luciferase in human cells. 3rd generation lentiviral vectorDepositorInsertLuciferase
UseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW13.lentiguide.mCherry
Plasmid#104375PurposemCherry vector for cloning in guidesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LRG2.1_Puro
Plasmid#125594PurposeLentiviral expression of sgRNA with GFP and puromycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for sgRNA expression and EFS promoter…Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a/b-actin
Plasmid#82583Purpose5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expressionDepositorInsertEF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-nSyb-IVS-PhiC31
Plasmid#133868PurposeReplace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.DepositorInsertPhiC31 Recombinase (int )
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX-RCC1-HA
Plasmid#106938PurposeTransient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)DepositorInserthuman RCC1-HA (RCC1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV promoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xHRE-MinTK-CRE-ODD
Plasmid#141147Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert4xHRE-MinTK-CRE-ODD
UseLentiviralTagsExpressionMutationcontains an altered Cre gene modified by the addi…PromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only