Skip to main content
Addgene
Showing: 4441 - 4460 of 37873 results

  1. BD-pGEX5X3-BD-SARS-CoV-2-S-tail-Thr1273Asp

    Plasmid
    #218457
    Purpose
    Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail (Thr1273Asp substitution) with an N-terminal GST tag
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    Cytosolic tail of SARS-CoV-2 spike carrying Thr1273Asp substitution and an N-terminal GST-tag (S SARS-CoV-2)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Thr1273Asp substitution
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218457-plasmid-map-sequence-id-436203

    Enlarge

  2. An R57A, R59A CAAX pMT2

    Plasmid
    #206119
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206119-plasmid-map-sequence-id-415935

    Enlarge

  3. An R57A, R59A HA CAAX pMT2

    Plasmid
    #206126
    Purpose
    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag
    Depositor
    Annette Dolphin
    Article
    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Oct. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    206126-plasmid-map-sequence-id-412911

    Enlarge

  4. CAGGS-safb∆dd1-NLS-3XFLAG-V5

    Plasmid
    #196092
    Purpose
    Expresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 96-285 (predicted disordered …
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196092-plasmid-map-sequence-id-384633

    Enlarge

  5. CAGGS-safb∆dd3-NLS-3XFLAG-V5

    Plasmid
    #196094
    Purpose
    Expresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 638-925 (predicted disordered…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196094-plasmid-map-sequence-id-384649

    Enlarge

  6. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid
    #196095
    Purpose
    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Contains only AA 619-925 (NLS and predicted disor…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196095-plasmid-map-sequence-id-384632

    Enlarge

  15. pDONR201-JIP60ml

    Plasmid
    #104959
    Purpose
    Encodes a modified, active, form of Hordeum vulgare c.v. Golden Promise Jasmonate Induced Protein 60 (JIP60)
    Depositor
    Pietro Spanu
    Article
    Pennington et et al Figshare (2018)
    Insert
    Jasmonate Induced Protein 60 (JIP60)
    Use
    Gateway entry vector for lr recombination into ot…
    Tags
    Expression
    Mutation
    Replaced amino acids 164 to 187 with a methionine…
    Promoter
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    104959-plasmid-map-sequence-id-204404

    Enlarge

  19. pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid
    #112668
    Purpose
    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression of your protein of interest.
    Depositor
    Catherine Verfaillie
    Article
    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-
    Insert
    tdT
    Use
    AAV; Donor plasmid for recombinase-mediated casse…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112668-plasmid-map-sequence-id-229324

    Enlarge

  20. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

    Enlarge

Showing: 4441 - 4460 of 37873 results