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Showing: 4441 - 4460 of 34792 results

  3. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid
    #170209
    Purpose
    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus
    Depositor
    Kelly Monk
    Article
    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.
    Insert
    slc1a3b (slc1a3b Zebrafish)
    Use
    Zebrafish
    Tags
    myrGCaMP6s-P2A-H2AmCherry
    Expression
    Mutation
    Promoter
    slc1a3b
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170209-plasmid-map-sequence-id-333268

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  7. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  8. pU6T7G

    Plasmid
    #71463
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)
    Depositor
    Willie Mark
    Article
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    71463-plasmid-map-sequence-id-128761

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  11. pMO746

    Plasmid
    #117480
    Purpose
    upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR
    Depositor
    Judy Wall
    Article
    Parks et al Science. 2013 Mar 15;339(6125):1332-5. d
    Insert
    uracil phosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    117480-plasmid-map-sequence-id-273029

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  15. CMV-GEX-GECO1

    Plasmid
    #32443
    Purpose
    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging
    Depositor
    Robert Campbell
    Article
    Zhao et al Science. 2011 Sep 8.
    Insert
    GEX-GECO1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…
    Promoter
    CMV
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 4441 - 4460 of 34792 results