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Plasmid#107974Purposeexpression of a dominant negative MAP Kinase KinaseDepositorInsertMKK1 K97A (MAP2K1 Human)
UseTagsExpressionMammalianMutationdominant negative; see Depositor Comments belowPromoterCMVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239D
Plasmid#22220DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239A
Plasmid#22219DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
Ai9
Plasmid#22799DepositorInsertCAG-Floxed tdTomato
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D12L
Plasmid#89161PurposeExpress vaccinia virus D12L in mammalian cellsDepositorInsertVaccinia virus D12L
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
nNOS/NOS1 scFv [L121/133]
Plasmid#206759PurposeMammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.DepositorInsertnNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-dADAM17 (#1)
Plasmid#177259PurposeA knockout vector for the dog Adam17DepositorInsertA gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE
Plasmid#138147PurposeExpresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
p4489 Flag-betaTrCP
Plasmid#10865DepositorInsertbetaTrCP (BTRC Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-SpyTag-sfCherry2(11)-TagBFP
Plasmid#117485PurposeExpresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cellsDepositorInsertSpyTag002-GG-sfCherry2(11)
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgAAVS1
Plasmid#192480PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as controlDepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-flex-Voltron-ST (AAV1)
Viral Prep#119036-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hsyn-flex-Voltron-ST (#119036). In addition to the viral particles, you will also receive purified pAAV-hsyn-flex-Voltron-ST plasmid DNA. Synapsin-driven, cre-dependent expression of genetically encoded fluorescent voltage indicator Voltron1 with a soma-targeting sequence. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mNeonGreen
Plasmid#98877PurposeIn vivo visualization of actin cytoskeleton (can be used for colocalization studies)DepositorArticleInsertLifeAct
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Soma-jGCaMP8m (AAV9)
Viral Prep#169257-AAV9PurposeReady-to-use AAV9 particles produced from AAV-hSyn-Soma-jGCaMP8m (#169257). In addition to the viral particles, you will also receive purified AAV-hSyn-Soma-jGCaMP8m plasmid DNA. Syn-driven expression of soma-targeted calcium sensor jGCaMP8m. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsNoneAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Brainbow3.2-PGK-PuroTK
Plasmid#159928PurposeBrainbow transgenesisDepositorInsertsNFPnls
mOrange2
EGFP
mKate2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only