Addgene

We narrowed to 21,248 results for: promoter

Showing: 4401 - 4420 of 21248 results

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pTrEno

Plasmid

#115474

Purpose

Eno-promoter driven expression vector for protein production in Trichoderma reesei

Depositor

Steve Decker

Article

Linger et al Biotechnol Biofuels. 2015 Mar 18;8:45. d

Insert

Cel7A

Use

Unspecified

Tags

Expression

Mutation

Introns have been removed

Promoter

Trichoderma reesei Enolase (Eno) promoter

Available since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p3e UbB polyA

Plasmid

#188702

Purpose

Gateway 3' element encoding the ubiquitin B polyadenylation sequence

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

UbB polyA (ubb Zebrafish)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-trc- YFP

Plasmid

#186569

Purpose

p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-A53T

Plasmid

#40823

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

A53T (aggregates more rapidly than the wild-type …

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.GFP

Plasmid

#57822

Purpose

3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
eGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM28

Plasmid

#124960

Purpose

Mammalian expression of Human TRIM28

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPML1 (GB0045)

Plasmid

#68164

Purpose

Provides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtML1 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-HTNC

Plasmid

#13763

Depositor

Klaus Rajewsky

Article

Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

Insert

TAT-NLS-Cre

Use

Baculovirus

Tags

His Tag, NLS, and TAT

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLX307 Luciferase

Plasmid

#117734

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Use

Tags

FLAG

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT

Plasmid

#1787

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA11-mRuby3

Plasmid

#184894

Purpose

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA11 (ANXA11 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

R230C relative to NP_001148.1

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131-tRNA2.0

Plasmid

#158393

Purpose

Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28c(+)-YTHDF3

Plasmid

#102276

Purpose

For bacterial expression of His-tagged human YTHDF3

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF3 (YTHDF3 Human)

Use

Tags

6X HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pHrD-IRES-Fluc

Plasmid

#194250

Purpose

Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

Depositor

Samson Jacob

Article

McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

human rDNA promoter and beginning of 5' ETS …

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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