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Showing: 4401 - 4420 of 11285 results

  6. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  12. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid
    #157691
    Purpose
    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157691-plasmid-map-sequence-id-307833

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  14. pcDNA3-FLAG-LplA(AAG)

    Plasmid
    #61307
    Purpose
    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
    Depositor
    Alice Ting
    Article
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Insert
    E. coli lipoic acid ligase
    Use
    Tags
    6xHis, Flag, T7 tag, and Xpress epitope
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61307-plasmid-map-sequence-id-99628

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  20. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154105-plasmid-map-sequence-id-304581

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Showing: 4401 - 4420 of 11285 results