Showing: 4401 - 4420 of 16077 results
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Plasmid#41825PurposeUsed as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.DepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7, TEFAvailabilityAcademic Institutions and Nonprofits only
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Antibody#184170-rAbPurposeAnti-LRRK2/Dardarin, C-terminus (Human) recombinant mouse monoclonal antibodyDepositorRecommended ApplicationsImmunocytochemistry, Immunohistochemistry, and Western BlotReactivityHuman, Mouse, and RatSource SpeciesMouseIsotypeIgG2aTrial SizeAvailable to purchaseAvailabilityIndustry, Academic Institutions, and Nonprofits
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pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pDS247
Plasmid#34967DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407A, V416I, T–2SPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDS248
Plasmid#34968DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407A, V416IPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDS250
Plasmid#34969DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationV416IPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDS255
Plasmid#34970DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationT406A, T407APromoterAvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA7-spo5DSR
Plasmid#49873Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKM495 (Flag-Das+4) Zeo
Plasmid#109284PurposeORBIT integrating plasmid for C-terminal tagging with degradation tag DAS+4, using zeocin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDNA3-Cerulean-FHA1-NES
Plasmid#138202PurposeEncodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.DepositorInsertCerulean-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AktAR-Kras
Plasmid#123357PurposeFRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.DepositorInsertAktAR-Kras
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET29b-eGFP-dspB(E184Q)-6xHis
Plasmid#175783PurposePlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and eGFPExpressionBacterialMutationE184QPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
UseTagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaPromoterAvailabilityAcademic Institutions and Nonprofits only -
KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193317PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
UseTags4FLAGExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Flag-nls-NgAgo-GK
Plasmid#86435PurposeExpresses a Flag tag on the N-terminalDepositorInsertNgAgo
UseTagsFlag tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pRFHUE-eGFP
Plasmid#89469PurposeGFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformationDepositorInserteGFP
UseBinary plasmid for atmt of filamentous fungiTagsExpressionMutationPromoterA. nidulans gpdAAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorInsertXRCC4 (XRCC4 Human)
UseTagsGFP-2xFLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmiRFP703-Tubulin
Plasmid#79991PurposeAlpha tubulin labeling with near-infrared fluorescent protein miRFP703DepositorInsertTubulin (TUBA1B Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 4401 - 4420 of 16077 results