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Plasmid#171803PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-gB-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-Ova-BlastR [M1G]
Plasmid#171808PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1GDepositorInsertmNeonGreen-F-Ova-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-gB-BlastR [M1G]
Plasmid#171807PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1GDepositorInsertmNeonGreen-F-gB-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-BlastR [M1G]
Plasmid#171806PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and blasticidin resistance cassette [p.M1G]DepositorInsertmNeonGreen-F-hgp100-T2A-BlastR
UseGene taggingTagsExpressionMutationBlastR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-Ova-PuroR [M1G]
Plasmid#171804PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-Ova-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-mgp100-PuroR [M1G]
Plasmid#171802PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-mgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2F04
Plasmid#70901PurposeGateway entry cloneDepositorInsertmod(mdg4) (mod(mdg4) Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVQ CMV NanoV1-2a-EGFP ferritin
Plasmid#79649PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVQ CMV NanoV1Mutant-2a-EGFP ferritin
Plasmid#79650PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralTagsExpressionMammalianMutationisoleucine 679 changed to lysinePromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-IVT
Plasmid#205145PurposeIVT template of AcGFP with MCS in frame and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-stop-IVT
Plasmid#205146PurposeIVT template of AcGFP stop codon between the ORF and MCS and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFFLuc-IVT
Plasmid#205147PurposeIVT template of FireFly luciferase stop codon between the ORF and MCS and polyADepositorInsertFireFly luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRLuc-IVT
Plasmid#205148PurposeIVT template of Renilla luciferase stop codon between the ORF and MCS and polyADepositorInsertRenilla Luciferase
UseLuciferase; In-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS_SN_400
Plasmid#170472PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, Os, O-, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYY_S2_400
Plasmid#170471PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, Os, O2, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYY_N1_400
Plasmid#170473PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O-, O1, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS_2S_400
Plasmid#170478PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O2, Os, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS_2N_400
Plasmid#170479PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O2, O-, Lambda t1 in lacZ fragment
UseTagsExpressionBacterialMutationPromoterT7A1Available sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLX-R4-DEST-R2
Plasmid#45956DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4-DEST-R2-PG
Plasmid#45957DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only