Addgene

We narrowed to 11,157 results for: cel.2

Showing: 4401 - 4420 of 11157 results

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LLP670_L2_pV01_0I_SloxM1-OCS-Rluc_lacZ

Plasmid

#192422

Purpose

To act as the off state for testing of the alt SCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::SloxM1-OCS-SloxM1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP672_L2_pV01_0I_VloxP-OCS-Rluc_lacZ

Plasmid

#192424

Purpose

To act as the off state for testing of the alt VCre recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::VloxP-OCS-VloxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP674_L2_pV01_0I_vox-OCS-Rluc_lacZ

Plasmid

#192426

Purpose

To act as the off state for testing of the alt Vika recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::vox-OCS-vox::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC

Plasmid

#192371

Purpose

To test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP760_L2pV1_OR-OCS_TCTP-Flp

Plasmid

#192419

Purpose

To act as a 1-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ

Plasmid

#192383

Purpose

To act as the off state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ

Plasmid

#192384

Purpose

To act as the on state for testing of the alt B3 recombinase.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::B3RT-OCS-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM408-5p

Plasmid

#84218

Purpose

Disturb the expression of miR408-5p in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR408-5p

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM530-3p

Plasmid

#84226

Purpose

Disturb the expression of miR530-3p in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR530-3p

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a - MPX

Plasmid

#89619

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Mitocondrial Tryparedoxin peroxidase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfB

Plasmid

#89617

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

electron-transfer-flavoprotein, alpha polypeptide

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TDR

Plasmid

#89627

Purpose

Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

thiol dependent reductase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - NFDD

Plasmid

#89624

Purpose

Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

NAD/FAD dependent dehydrogenase

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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M35 Dpr yeast clone

Plasmid

#17191

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Tags

Gal4-AD

Expression

Yeast

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
M36 Dpr C-terminal GST

Plasmid

#17192

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr (dact1-b Frog)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
T7-VEE-GFP

Plasmid

#58977

Purpose

expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

EGFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

26S subgenomic promoter

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRV.GFP FOXP3

Plasmid

#13250

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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