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pActin5c-CD-dCas9m4-UDI-attB
Plasmid#104879PurposeTo generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9DepositorInsertCD-dCas9(m4)-UDI
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR-mRho.V5.mER.hOr56a
Plasmid#126479PurposeHuman codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cellsDepositorUseTagsH. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…ExpressionMammalianMutationcodon optimization for H. sapiens and codon optim…PromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry
Plasmid#159631Purposeexpresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoterDepositorInsertAst (AstA Fly)
UseLentiviralTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2F04
Plasmid#70901PurposeGateway entry cloneDepositorInsertmod(mdg4) (mod(mdg4) Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-Cdh1wt (570)
Plasmid#39877DepositorInsertFZR1 (FZR1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDmelOR-mRho.V5.mER.hOr47a
Plasmid#126478PurposeHuman codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cellsDepositorUseTagsH. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…ExpressionMammalianMutationcodon optimization for H. sapiens and codon optim…PromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE
Plasmid#159630PurposeAAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoterDepositorInsertAst (AstA Fly)
UseAAVTagsHA, IRES, mCherry, and neurophysin-1ExpressionMammalianMutationPromoterDCA (same as CAG promoter)Available sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)
Plasmid#102277PurposeFor expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)DepositorInsertYTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
UseFor mammalian cell-free protein expression system…TagsGST, HA, and HISExpressionMutationPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-FLuc-V5His6
Plasmid#21183DepositorInsertFirefly Luciferase
UseDrosophila expressionTagsExpressionMutationPromoterAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET11a-dH2A
Plasmid#105500PurposeExpression of Drosophila histone H2ADepositorInsertdH2A
UseTagsExpressionMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUAST-attB-GFP-V5-His
Plasmid#85621PurposeExpresses GFP and V5 in Drosophila.DepositorInsertGFP
UseTagsV5, HisExpressionInsectMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-NDH6
Plasmid#105503PurposeExpression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tagDepositorInsertNDH6
UseTags6xHisExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQF2wWB
Plasmid#61313PurposeEnhancer trap version of QF2w, analogous to pGAWBDepositorInsertQF2w
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQF2WB
Plasmid#61312PurposeEnhancer trap version of QF2, analogous to pGAWBDepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHSS6hsILMi20
Plasmid#102536PurposeHelper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)DepositorInsertMinos transposase
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-His6dNLP
Plasmid#105498PurposeExpression of N-terminally 6His tagged Drosophila nucleoplasmin-like proteinDepositorInsertHis6dNLP
UseTagsExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sall4
Plasmid#13361DepositorInsertsal-like 4 (Drosophila) (Sall4 Mouse)
UseRetroviralTagsExpressionMammalianMutation*(see below)PromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only
