Addgene

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JPF0419v

Plasmid

#124021

Purpose

Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

Plasmid

#139482

Purpose

Expresses PhyB621-mCherry-HRasCT in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

PhyB621 (PHYB Mustard Weed)

Use

Lentiviral

Tags

mCherry-HRasCT

Expression

Mammalian

Mutation

G229L and deletion of aa Y at position 235 in mch…

Promoter

Tet responsible element

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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MigR1-Myc-Nterm TRIB2

Plasmid

#131153

Purpose

Retroviral expression of N terminus of mouse TRIB2

Depositor

Warren Pear, Karen Keeshan

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 1-145

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUDR220

Plasmid

#113873

Purpose

expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

Depositor

Jack Pronk, Jean-Marc Daran

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA8-HXT10 sgRNA7-HXT9-11-12

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCOG75-Sem1 Sc

Plasmid

#83233

Purpose

pCDF-His6-MBP-Sem1 Sc

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

SEM 1 (SEM1 Budding Yeast)

Use

Tags

His-mbp

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX-HA

Plasmid

#14987

Depositor

Michael Ristow

Article

Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

Insert

human frataxin cDNA (FXN Human)

Use

Tags

AU1 and HA

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pCR3-vsv-AuroraB L154G/H250Y

Plasmid

#108490

Purpose

expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (sense)

Plasmid

#83816

Purpose

Mammalian expression of mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3 (Gata3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
STARR-seq luciferase validation vector_mP_CMV

Plasmid

#99313

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Use

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
MT-AvrXa10-FT

Plasmid

#234373

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

Depositor

Michael Smanski

Article

Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

Inserts

NbGP41-GFP-AvrXa10-GB1
dCas9-24XGP41
gRNAs targeting FT locus
RFP

Use

Synthetic Biology

Tags

GB1, GFP, and GP41

Expression

Plant

Mutation

Promoter

Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#229135

Purpose

Retrovirus driving mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

Plasmid

#225353

Purpose

Lentiviral vector for DKK1 and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Dkk1 (Dkk1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

Plasmid

#225355

Purpose

Lentiviral vector for ActivinA and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Actvin A and TagBFP (Inhba Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Human, Synthetic)

Use

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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