Addgene

We narrowed to 9,017 results for: yeast

Showing: 421 - 440 of 9017 results

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pHyg-AID*-6FLAG

Plasmid

#99519

Purpose

C-terminal AID*-6FLAG degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xFLAG

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

Plasmid

#179079

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHyg-AID*-6HA

Plasmid

#99520

Purpose

C-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

6xHA

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCASB

Plasmid

#190175

Purpose

Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

Depositor

Jessica Tyler

Article

Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

Insert

sgRNA cloning site

Use

Tags

Expression

Bacterial and Yeast

Mutation

Added 5'-agagacc-3' upstream and 5'…

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pHis-AID*-9myc

Plasmid

#99524

Purpose

C-terminal AID*-9myc degron cassette with HIS3MX marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-AID*-9myc

Plasmid

#99518

Purpose

C-terminal AID*-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

Plasmid

#179078

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-AID*-GFP

Plasmid

#99521

Purpose

C-terminal AID*-GFP degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

GFP

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFPI7

Plasmid

#83555

Purpose

Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CUP1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJH001

Plasmid

#67641

Purpose

High copy Cas9 expression vector with LEU2 marker for yeast transformation

Depositor

John Wyrick

Article

Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

Inserts

Cas9
Empty

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP91

Plasmid

#83548

Purpose

Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PGK1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP92

Plasmid

#83549

Purpose

Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TDH3 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB6364

Plasmid

#106151

Purpose

EasyCloneYALI system-based yeast integrative vector expressing Cas9 carrying D-serine marker, integration into Yarrowia lipolytica ku70 locus, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

Cas9

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFPB5A

Plasmid

#83556

Purpose

Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

GAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pILGFP97

Plasmid

#83551

Purpose

Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CYC1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJS288

Plasmid

#200714

Purpose

To express yeast Hxt1 hexose transporter in yeast

Depositor

Jens Boch

Article

Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

Inserts

PMA1 promoter (PMA1 Budding Yeast)
Hxt1 (HXT1 Budding Yeast)
ADH2 Ter (ADH2 Budding Yeast)

Use

Synthetic Biology

Tags

mCherry

Expression

Yeast

Mutation

domesticated for MoClo cloning (removal of intern…

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge