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Plasmid#120028PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLpC-L-IFP-F[1] (mammalian)
Plasmid#52901PurposeBinary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseRetroviralTagsExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLpC-L-IFP-F[2] (mammalian)
Plasmid#52902PurposeBinary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseRetroviralTagsExpressionMammalianMutationPromoterUnknownAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
doraWT_rescue_construct
Plasmid#190609PurposePuromycin-selectable expression of Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTagsExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P08
Plasmid#120019PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3DepositorInsertmClover3
UsePcr templateTagsmClover3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHJ18
Plasmid#48357PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiTagsExpressionMutationPromoterAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P04
Plasmid#120015PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1DepositorInsertmTFP1
UsePcr templateTagsmTFP1ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE
Plasmid#140087PurposeBackbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistanceDepositorInsertLbCpf1 (LbCas12a) crRNA backbone
UseLentiviralTagsEF1a-PuroExpressionMammalianMutationPromoterU6Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQC V5 HuR.wt IRES Puro
Plasmid#110386PurposeÎł-Retroviral transfer vector for expressing HuR (wild type), IRES-driven Puromycin selection.DepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA-iRPT
Plasmid#140763PurposeExpresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassetteDepositorInsertsiRFP713
PuroR
rtTAM2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
UseTagsExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P21
Plasmid#120032PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTagDepositorInsert24xSunTag
UsePcr templateTags24xSunTagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P03
Plasmid#120014PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3DepositorInsertmoxCerulean3
UsePcr templateTagsmoxCerulean3ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro
Plasmid#195567PurposePiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cellsDepositorInsertTIR1
UseTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLucIPZ
Plasmid#228703PurposeConstitutive expression of Gaussia luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsGaussia luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-doraWT_rescue_construct
Plasmid#190608PurposePuromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationPromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato166/892
Plasmid#179919PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiguide-puro_hNKD2sg#3
Plasmid#174325PurposegRNA targeting hNKD2 - encodes puromycin resistanceDepositorInserthuman NKD2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only