Addgene

We narrowed to 1,626 results for: nps

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vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Use

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Ub[9]-mRFP[1]

Plasmid

#20249

Depositor

Nico Dantuma

Article

Salomons et al Mol Cell Biol. 2009 Jan 21. ():.

Insert

ubiquitin C (UBC Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

changed Stop codon 686 to Glycine

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23117

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1)

Plasmid

#80049

Purpose

Expression of EPN-01 mutated in TSG101 binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159672

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

βTub-NLS-hSpCas9-T2A-GFP

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Use

Tags

T2A-GFP

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔM)

Plasmid

#80040

Purpose

Expression or G2A mutant of EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(G2A)

Use

Tags

Myc

Expression

Mammalian

Mutation

Gly2 mutated to Ala

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR[traF]+Opie2-PuroR[dsxM]

Plasmid

#131618

Purpose

Expresses a functional NeoR only in females, a functional PuroR only in males, and dsRed in both genders.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

PuroR[dsxM]
NeoR[traF]

Use

Tags

Expression

Insect

Mutation

Promoter

Opie2

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Tags

Expression

Mutation

Promoter

dU6-3

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL2)

Plasmid

#80050

Purpose

Expression of EPN-01 mutated in ALIX binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaYP)

Use

Tags

Myc

Expression

Mammalian

Mutation

YP37AA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL1)

Plasmid

#80042

Purpose

Expression of PTAP L domain mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

pCMV-EPN-01*(ΔL2)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP sequence in p6 mutated to Ala

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL2)

Plasmid

#80043

Purpose

Expression of YPxL L domain mutantd EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Use

Tags

Myc

Expression

Mammalian

Mutation

YP37 in p6 mutated to AA

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_1992_F

Plasmid

#237358

Purpose

Expression plasmid for RSV B F from 1992

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B F from 1992 human codon optimized from Genbank Accesion OK649748

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_OR795252_F_N201S

Plasmid

#237363

Purpose

Expression plasmid for RSV B F from OR795252

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B F human codon optimized from Genbank Accesion OR79525

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A_1982_F

Plasmid

#237355

Purpose

Expression plasmid for RSV A F from 1982

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV A F from 1982 human codon optimized from Genbank Accesion KJ723472

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_1982_F

Plasmid

#237357

Purpose

Expression plasmid for RSV B F from 1982

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B F from 1982 human codon optimized from Genbank Accesion MG642043

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_G_31AACTdel

Plasmid

#237350

Purpose

Expression plasmid for RSV Long G

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV Long G from Genbank Accesion AY911262.1

Use

Tags

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_2019_F

Plasmid

#237359

Purpose

Expression plasmid for RSV B F from 2019

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B F from 2019 human codon optimized from Genbank Accesion PP352433

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_B_MN365362_F_K68Q

Plasmid

#237361

Purpose

Expression plasmid for RSV B F from MN365362

Depositor

Jesse Bloom

Article

Simonich et al bioRxiv 2025.03.11.642476; doi: 10.1101/

Insert

RSV B F human codon optimized from Genbank Accesion MN36536

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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