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Plasmid#116890Purposeexpresses Gag missing the p6 domain, tagged with mEGFP, in mammalian cellsDepositorInsertsynthetic Gag missing the p6 domain
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
BiDi-[mp-mCherry2]mp-mEGFP
Plasmid#182871PurposeBi-directional expression vector of monomers with plasma membrane localization signalDepositorInsertmyr-palm-mCherry2, myr-palm-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mEYFP-(L)-mCherry2-(L)-mEGFP
Plasmid#182861PurposeHetero-trimer expression vectorDepositorInsertmEYFP-mCherry2-mEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP-mEGFP-FRET
Plasmid#58181PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmEGFP and mTagBFPExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEGFP-30nmSAH-DmrA
Plasmid#61680PurposeGFP-labeled SAH scaffold with DmrA attachment siteDepositorInsertmEGFP-30nmSAH-DmrA
UseTagsExpressionMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(-)-SEC61B-mEGFP
Plasmid#115921Purposedonor to insert mEGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-N1 YAPS127A- L325E
Plasmid#166464PurposeExpresses fusion of mEGFP and YAPS127A- L325EDepositorInsertmEGFP, YAPS127A- L325E (YAP1 Human)
UseTagsExpressionMammalianMutationYAPS127A- L325EPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mEGFP(3x)
Plasmid#182872PurposeHomo-trimer expression vector with plasma membrane localization signalDepositorInsertmyr-palm-mEGFP(3x)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-PIF3-mEGFP
Plasmid#139483PurposeExpresses PIF3-mEGFP in mammalian cells.DepositorInsertPIF3 (PIF3 Mustard Weed)
UseLentiviralTagsmEGFPExpressionMammalianMutationPIF3 1-100 aaPromoterHuman EF1a promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-mEGFP
Plasmid#115911Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorInsertTOMM20-mEGFP (TOMM20 Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPExpressionMutationPromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mEGFP
Plasmid#105794PurposeExpression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex0)
Plasmid#163429PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mEGFP/FRT/TO
Plasmid#27762DepositorInsertcaveolin-1 (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
Nup54-mEGFP494(flex2)
Plasmid#163431PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(K8,9A)-mEGFP
Plasmid#168490PurposeMammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.DepositorInsertthe K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-30nmSAH-GFP(1-10)
Plasmid#61677PurposeGFP-labeled SAH scaffold with split GFP attachment siteDepositorInsertmEGFP-30nmSAH-GFP(1-10)
UseTagsExpressionMutationPromoterAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-Rx3(A6)-mEGFP
Plasmid#35530DepositorInsertRx3(A6)mEGFP
UseRetroviralTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2012AvailabilityAcademic Institutions and Nonprofits only