Addgene

We narrowed to 3,798 results for: golden gate

Showing: 421 - 440 of 3798 results

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pGGEV_7'_XcmI-LacZ

Plasmid

#49308

Purpose

Golden Gate entry vector at position 7, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_8'_XcmI-LacZ

Plasmid

#49309

Purpose

Golden Gate entry vector at position 8, LacZ, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_-1_FRT_+1_OA-

Plasmid

#49310

Purpose

Golden Gate entry vector: Position 1, FRT site

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

FRT forward

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_3_FRT_+1_OA-

Plasmid

#49311

Purpose

Golden Gate entry vector: Position 3, FRT site

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

FRT forward

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_2'_Linker

Plasmid

#49288

Purpose

Golden Gate entry vector at position 2, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_4'_Linker

Plasmid

#49290

Purpose

Golden Gate entry vector at position 4, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_6'_Linker

Plasmid

#49292

Purpose

Golden Gate entry vector at position 6, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_7'_Linker

Plasmid

#49293

Purpose

Golden Gate entry vector at position 7, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGGEV_8'_Linker

Plasmid

#49294

Purpose

Golden Gate entry vector at position 8, small linker insert, final entry vector for the assembly

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_XcmI-LacZ

Plasmid

#49295

Purpose

Golden Gate entry vector at position 1, plus 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL30008

Plasmid

#50300

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_US71_APikL1

Plasmid

#123646

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

US71_APikL1

Use

Golden gate

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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GGE114

Plasmid

#120731

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Type

Empty backbone

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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LI F-G Spec

Plasmid

#104521

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Spectinomycin resistance gene

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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GGE151

Plasmid

#120751

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_4UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GGE152

Plasmid

#120752

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_8UASxpr-TEF)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P2_8UASxpr-TEF

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Article

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Expression

Mutation

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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