Addgene

We narrowed to 3,275 results for: aaas

Showing: 421 - 440 of 3275 results

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pFL His-SDS22 / PP1gamma

Plasmid

#113511

Purpose

Expression of His-SDS22 / PP1gamma dimer in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Inserts

SDS22 (PPP1R7 Human)
PP1gamma (PPP1CC Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-mEos3.2-I3

Plasmid

#113516

Purpose

Expression of His-Eos-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His-mEos3.2

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Use

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 UBXN2A

Plasmid

#113505

Purpose

Bacterial expression of GST-tagged UBXN2A

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

UBXN2A (UBXN2A Human)

Use

Tags

GST-Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_15aa

Plasmid

#23969

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_15aa (UBB Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO PP1gamma-2*Strep

Plasmid

#113506

Purpose

Expresses dual Strep-tagged PP1gamma in mammalian cells

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

PP1gamma (PPP1CC Human)

Use

Tags

Strep-Strep

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*

Plasmid

#195220

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

H477/R479/S481>AAA

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*/PBM*

Plasmid

#195221

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. Dual mutant for HRS>AAA / lacking DTHL.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Dual mutant for HRS>AAA / lacking DTHL

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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deltaLoop-MIDAS

Plasmid

#171605

Purpose

Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

Depositor

Tarun Kapoor

Article

Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)

Use

Tags

His6 tag and SNAP tag

Expression

Bacterial

Mutation

Replaced amino acids 4458-4496 with ASGSGS linker

Promoter

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-His D592Amb

Plasmid

#169022

Purpose

Expresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His

Expression

Bacterial

Mutation

D592TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Use

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFL I3

Plasmid

#113514

Purpose

Expression of I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-I3 V41A / W43A

Plasmid

#113515

Purpose

Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

V41A /W43A

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37deltaSEP

Plasmid

#113501

Purpose

Bacterial expression of GST-tagged p37 lacking the SEP domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 140-206

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37delta2-81

Plasmid

#113502

Purpose

Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 2-81

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFL His-I3

Plasmid

#113513

Purpose

Expression of His-I3 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

I3 (PPP1R11 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 1

Plasmid

#8470

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

194YDC196-AAA (Y194A, D195A, C196A)

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
HA Raptor mutant 2

Plasmid

#8471

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Use

Tags

ha

Expression

Mammalian

Mutation

261DLF263-AAA (D261A, L262A, F263A)

Promoter

Available since

May 11, 2005

Availability

Academic Institutions and Nonprofits only
pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Use

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only