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Plasmid#214395PurposemAID-dTAG for C-terminal taggingDepositorInsertmAID-dTAG-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK486 (Hygro-P2A-2mAID)
Plasmid#214398Purpose2mAID for C-terminal taggingDepositorInsertHygro-P2A-2mAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK487 (Hygro-P2A-3mAID)
Plasmid#214399Purpose3mAID for C-terminal taggingDepositorInsertHygro-P2A-3mAID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK382
Plasmid#214373PurposeTet-OsTIR1(F74G) (AAVS1 insertion donor)DepositorInsertTet-OsTIR1(F74G)
UseTagsExpressionMammalianMutationPromoterTet ON 3G promoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK450 (BromoTag-mCherry-BSD)
Plasmid#214380PurposeBromoTag-mCherry for N-terminal taggingDepositorInsertBromoTag-mCherry-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK451 (BSD-P2A-dTAG)
Plasmid#214381PurposedTAG for N-terminal taggingDepositorInsertBSD-P2A-dTAG
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK452 (BSD-P2A-dTAG-mClover)
Plasmid#214382PurposedTAG-mClover for N-terminal taggingDepositorInsertBSD-P2A-dTAG-mClover
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK455 (dTAG-mClover-BSD)
Plasmid#214385PurposedTAG-mClover for C-terminal taggingDepositorInsertdTAG-mClover-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK456 (dTAG-mCherry-BSD)
Plasmid#214386PurposedTAG-mCherry for C-terminal taggingDepositorInsertdTAG-mCherry-BSD
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK464 (mAB-Neo)
Plasmid#214390PurposemAID-BromoTag for C-terminal taggingDepositorInsertmAID-BromoTag-Neo
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM045
Plasmid#217399PurposeKmR, pBTRcK broad host range vector with BTE1(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE1
UseTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM046
Plasmid#217400PurposeKmR, pBTRcK broad host range vector with BTE2(C12-specific fatty acid thioesterase codon-optimised for C. necator) under PtrcDepositorInsertBTE2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPTrcAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS161
Plasmid#215683PurposeGuide only plasmid targeting chrIII split hygromycinR landing padDepositorInsertU6p::GTCCAGCGGCAGATCGGCGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS160
Plasmid#215682PurposeGuide only plasmid targeting chrI split hygromycinR landing padDepositorInsertU6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA2.0
Plasmid#208299PurposeExpresses ABE-RA2.0 in mammalian cellsDepositorInsertTadA-RA2.0-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, D108G, K110R, H122R, M126I, N12…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.0
Plasmid#208301PurposeExpresses ABE-RA3.0 in mammalian cellsDepositorInsertTadA-RA3.0-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.1
Plasmid#208302PurposeExpresses ABE-RA3.1 in mammalian cellsDepositorInsertTadA-RA3.1-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, R47K, P48A, R51H, I76F, D108G, K110R, H122R…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.2
Plasmid#208303PurposeExpresses ABE-RA3.2 in mammalian cellsDepositorInsertTadA-RA3.2-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, P48A, R51H, D108G, K110R, A114V, H122R, M12…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA3.3
Plasmid#208304PurposeExpresses ABE-RA3.3 in mammalian cellsDepositorInsertTadA-RA3.3-SpCas9 D10A
UseTagsExpressionMammalianMutationE27D, R47K, P48A, R51H, I76F, D108G, K110R, A114V…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA4.0
Plasmid#208305PurposeExpresses ABE-RA4.0 in mammalian cellsDepositorInsertTadA-RA4.0-SpCas9 D10A
UseTagsExpressionMammalianMutationP48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only