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Plasmid#72575PurposeExpresses shRNA against PICK1 as well as the marker Venus fluorescent proteinDepositorUseTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only
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Scarlet I-FRET 10-Venus
Plasmid#139366PurposeFRET StandardDepositorInsertScarlet 10AA Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.A184V
Plasmid#106196PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterGFAPAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.S72A
Plasmid#106197PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterGFAPAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-PKCB1 K371R
Plasmid#195230PurposeRetroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.DepositorInsertPKCB1 (PRKCB Human)
UseRetroviralTagsExpressionMutationK371RPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-Venus-His8
Plasmid#223889Purpose4G-cloning donor for C-terminal 3C-Venus-His8 tagDepositorInsert3C-Venus-His8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-OEP7-mVenus
Plasmid#213864PurposeBinary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.DepositorInsertchloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
mVenus-Cyclin D1-NeoR
Plasmid#215068PurposeExpresses mVenus-Cyclin D1 in mammalian cells.DepositorInsertCCND1 (CCND1 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3
Plasmid#209090PurposemVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:γB2-EC5-VenusΔN3C9-P3
Plasmid#209092PurposemVenus-inserted PcdhgB2DepositorInsertPcdhgB2 (Pcdhgb2 Mouse)
UseTagsPA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:TDimer mVenus
Plasmid#210520PurposeExpresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertTandem dimer mVenus
UseZebrafish expression, tol2 kit gateway-compatibleTagsExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:R198P mVenus
Plasmid#210519PurposeExpresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationR198PPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2-Venus
Plasmid#203712PurposeConstitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent proteinDepositorInsertNKX6-2 fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NKX6-2(1-40)-Venus
Plasmid#203713PurposeSPAX8-related truncated mutation (K41*) containing the amino acids 1 to 40 of human NKX6-2, fused with the Venus fluorescent protein.DepositorInsertNKX6-2 (1-40) fused with Venus (NKX6-2 Human)
UseTagsVenusExpressionMammalianMutationdeletion of amino acids 41-277PromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pVRC-N-Venus-V150A-CC-Di
Plasmid#190707PurposeEncodes a modified version (V150A) of N-Venus followed by the parallel coiled-coil CC-Di.DepositorInsertN-Venus-V150A-CC-Di
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A
Plasmid#190708PurposeControl plasmid encoding a modified version (V150A) of N-Venus, but without a coiled-coil to drive reconstitution.DepositorInsertN-Venus-V150A
UseTagsExpressionMutationV150APromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-C-Venus-apCC-Di
Plasmid#190711PurposeEncodes C-Venus followed by the antiparallel coiled-coil apCC-Di.DepositorInsertC-Venus-apCC-Di
UseTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only