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Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P), DHFR (P66L)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
UseTagsExpressionYeastMutationGAL3 (S509P), DHFR (P66L)PromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRE-8XGTIIC-DsRED-DD
Plasmid#115798Purposelentiviral, trimethoprim (TMP) regulated YAP promoter activity reporterDepositorInsertYAP responsive promoter (YAP1 Human)
UseLentiviralTagsDsRED-DHFRExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pERB260
Plasmid#67763PurposeExpresses BICD-mCherry-eDHFRDepositorInsertBICD-mCherry-eDHFR
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP-LUC
Plasmid#60976PurposeRapid selection of recombinant marker-free bioluminescent rodent malaria parasitesDepositorInsertsGFP-Luciferase fusion
mCherry
hDHFR-yFCU
UseTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GOMO-GFP
Plasmid#60975PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasitesDepositorInsertsGFP
mCherry
hDHFR-yFCU
UsePlasmodium gene targetingTagsExpressionMutationPromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)
Plasmid#117911PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A & H45Q on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
UseTagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTP1
Plasmid#64952Purposesource of TpR FRT cassetteDepositorInsertFRT-dhfrII-FRT
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
10xUAS-GFP-DD
Plasmid#104989PurposeExpresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized GFP (GFP-DD)
UseTagsDDExpressionInsectMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
10XUAS-flp-DD
Plasmid#124777PurposeExpresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.DepositorInsertTMP-inducible destabilized FLP (FLP-DD)
UseTagsDDExpressionInsectMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
nSYB-GAL80-DD
Plasmid#124778PurposeExpresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoterDepositorInsertTMP-inducible destabilized GAL80 (GAL80-DD)
UseTagsDDExpressionInsectMutationPromoternSynaptobrevinAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
PL6-CS-WR
Plasmid#122512Purposeconstruction for sgRNA transcription with Plasmodium falciparum U6 promotorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterPfU6Available sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-F98 (for GFP)
Plasmid#130714PurposePlasmid encoding the bacterial expression of LAMA-F98 for GFPDepositorInsertLAMA-F98
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98
Plasmid#130704PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-F98
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only