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Plasmid#228827PurposeFor the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC C-terminally fused to a mitochondrial targeting sequence in HeLa Flp-In cellsDepositorInsertScaC
UseTagsMTS and eGFPExpressionMammalianMutationaa 33-223PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4652
Plasmid#215266PurposeTU for the copper regulated expression of the flippase (FLP) recombinase.DepositorInsertCBS:miniDFR:FLP:TNOS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ConVERGD-eGFP-W3SL
Plasmid#218748PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO FLAG-RPS28
Plasmid#203156PurposeN-FLAG-RPS28 in inducible pcDNA5 vector for Flp-In integrationDepositorInsertRPS28 (RPS28 Human)
UseTags1X FLAG tag with GGGGS linkerExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-Cdc42
Plasmid#163606PurposeExpresses Cdc42 Flp-dependently in mammalian cellsDepositorInsertCdc42 (Cdc42 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-eGFP-WPRE
Plasmid#203843PurposeFlp-dependent EGFP expressionDepositorInsertEGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-N2cG-WPRE
Plasmid#218757PurposeProvides AND intersectional (Cre+Flp) expression of N2C glycoproteinDepositorInsertN2cG
UseAAVTagsExpressionMutationSee Depositor CommentsPromoterhSynAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-mCherryP2ACre
Plasmid#161773PurposeFlp-Dependent mCherry and Cre expressionDepositorInsertmCherry-P2A-Cre
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc
Plasmid#63167PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporterDepositorInsert"spaghetti monster GFP-cMyc"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC208-10XUAS-FRT>STOP>FRT-myr::smGFP-FLAG
Plasmid#63169PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-FLAG reporterDepositorInsert"spaghetti monster GFP-FLAG"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-stGtACR1(FusionRed)-W3SL
Plasmid#218761PurposeProvides AND intersectional (Cre+Flp) expression of stGtACR1-FusionRedDepositorInsertstGtACR1
UseAAVTagsFusionRedExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FRT-EF1a-TVA-2A-N2cG
Plasmid#172361PurposeTargeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neuronsDepositorInsertTVA + B19-N2cG
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL
Plasmid#218759PurposeProvides AND intersectional (Cre+Flp) expression of HA-hM4DiDepositorInserthM4Di
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL
Plasmid#218756PurposeProvides NOT intersectional (Cre NOT Flp) expression of TVA-mCherryDepositorInsertTVA-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL
Plasmid#218755PurposeProvides NOT intersectional (Flp NOT Cre) expression of TVA-mCherryDepositorInsertTVA-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-(HA)hM3Dq-W3SL
Plasmid#218758PurposeProvides AND intersectional (Cre+Flp) expression of HA-hM3DqDepositorInserthM3Dq
UseAAVTagsHAExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5_NLS-Halo-eGFP-BioID
Plasmid#218448Purposeexpresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integrationDepositorInsertNLS-Halo-eGFP-BirA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-EGFP-KASH
Plasmid#154374PurposeVector for Flp-dependent expression of EGFP tagged KASH protein domainDepositorInsertEGFP-KASH
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRT-KanR-FRT pA
Plasmid#82601Purpose3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selectionDepositorInsertFRT-Kan-FRT
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]
Plasmid#71809PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE
Plasmid#218760PurposeProvides AND intersectional (Cre+Flp) expression of ArchT-eGFPDepositorInsertArchT
UseAAVTagseGFPExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-dAPEX2
Plasmid#117175PurposePeroxidase reporter for multiplexed electron microscopy labelingDepositorInsertdAPEX2
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-tdTomato
Plasmid#125575PurposeFlp-dependent expression of tdTomato in mammalian cells.DepositorInserttdTomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS
Plasmid#63170PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporterDepositorInsert"spaghetti monster GFP-OLLAS"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]
Plasmid#71810PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV1-EF1α-F-Flex-jGCaMP7b-WPR
Plasmid#171689PurposeFlp-dependent expression of jGCaMP7bDepositorInsertGCaMP7b
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pADH100-2
Plasmid#90981PurposeNAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.DepositorInsertNAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
JPZF4 CMV-TO-SCN1A-383-NZF
Plasmid#236165PurposePlasmid encoding the SCN1A-383 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-383-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF5 CMV-TO-SCN1A-472-NZF
Plasmid#236166PurposePlasmid encoding the SCN1A-472 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-472-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EK0007-PB-4XnrUAS-H2B-BFP
Plasmid#236121PurposeFuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterE1b minimal promoter with four Gal4-binding sites…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF9 CMV-TO-SCN1A-371-NZF
Plasmid#236170PurposePlasmid encoding the SCN1A-371 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-371-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW409 ZNF250tarv3 x8-H2B-GFP
Plasmid#236133PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF250 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight ZNF250 binding si…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF2 CMV-TO-SCN1A-374-NZF
Plasmid#236163PurposePlasmid encoding the SCN1A-374 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-374-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF1 CMV-TO-SCN1A-377-NZF
Plasmid#236152PurposePlasmid encoding the SCN1A-377 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-377-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW410 IKZF1tar x8-H2B-GFP
Plasmid#236156PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight IKZF1 binding sit…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF6 CMV-TO-SCN1A-114-NZF
Plasmid#236167PurposePlasmid encoding the SCN1A-114 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-114-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
JPZF7 CMV-TO-SCN1A-140-NZF
Plasmid#236168PurposePlasmid encoding the SCN1A-140 zinc finger array attached by a GS linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertSCN1A-140-NZF
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only