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Showing: 4361 - 4380 of 21248 results

  1. pIVTR(AG)-TDsmURFP

    Plasmid
    #172307
    Purpose
    In vitro transcription of TDsmURFP
    Depositor
    Patrik Verstreken
    Insert
    TDsmURFP
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter A-initiating
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172307-plasmid-map-sequence-id-340448

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  2. pZP222_INTACT_AT

    Plasmid
    #225992
    Purpose
    To make INTACT lines under desired promoter
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    Tags
    INTACT
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    225992-plasmid-map-sequence-id-452361

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  3. SIN40C.EFS.eGFP.pre

    Plasmid
    #169276
    Purpose
    Constitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169276-plasmid-map-sequence-id-337928

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  4. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Insert
    humanized dCas13d
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  5. p300 core-dCas9-CBP core

    Plasmid
    #179559
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179559-plasmid-map-sequence-id-354771

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  6. pAAV-U6-racRNA-A

    Plasmid
    #200824
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200824-plasmid-map-sequence-id-396714

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  7. pAAV-U6-racRNA-B

    Plasmid
    #200825
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200825-plasmid-map-sequence-id-396771

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  8. pAAV-U6-racRNA-C

    Plasmid
    #200826
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200826-plasmid-map-sequence-id-396715

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  9. pAAV-U6-racRNA-D

    Plasmid
    #200827
    Purpose
    AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter
    Depositor
    Xiao Wang
    Article
    Shi et al Nature. 2023 Oct;622(7983):552-561. doi:
    Inserts
    U6-racRNA
    V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
    Use
    AAV
    Tags
    C-Far, Flag, and V5
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    200827-plasmid-map-sequence-id-396773

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  10. GCN5 FL-dCas9-CBP core

    Plasmid
    #179556
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179556-plasmid-map-sequence-id-354745

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  11. GCN5 core-dCas9-CBP core

    Plasmid
    #179555
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179555-plasmid-map-sequence-id-354784

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  12. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  13. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  14. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  15. pSYC-204

    Plasmid
    #178964
    Purpose
    Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Seok-Yong Choi
    Article
    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    R79C
    Promoter
    Zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178964-plasmid-map-sequence-id-356606

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  16. pSYC-201

    Plasmid
    #178963
    Purpose
    Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination
    Depositor
    Seok-Yong Choi
    Article
    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1
    Inserts
    Glial fibrillary acidic protein promoter (gfap Zebrafish)
    Glial fibrillary acidic protein (GFAP Human)
    EGFP-pA
    Use
    Tol2 mediated recombination
    Tags
    3xFLAG
    Expression
    Mutation
    Promoter
    zebrafish gfap promoter
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178963-plasmid-map-sequence-id-356608

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  17. CBP core-dCas9-p300 core

    Plasmid
    #179558
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of human CBP core (aa 1084-1701) & c-terminal fusion of human p300 core (aa 1048-1664) (CREBBP, EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179558-plasmid-map-sequence-id-354773

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  18. GCN5 FL-dCas9-p300 core

    Plasmid
    #179554
    Purpose
    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179554-plasmid-map-sequence-id-354754

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  19. FSW-tdTomato

    Plasmid
    #197033
    Purpose
    Express tdTomato from hSyn1 promoter
    Depositor
    Ronald Hart
    Insert
    tdTomato
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    197033-plasmid-map-sequence-id-386341

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  20. pAK_Tol2_TRE_Neo

    Plasmid
    #130262
    Purpose
    Tol2 doxycycline inducible expression vector
    Depositor
    Michael Yaffe
    Type
    Empty backbone
    Use
    Tol2 transposon, doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130262-plasmid-map-sequence-id-310816

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Showing: 4361 - 4380 of 21248 results