-
Plasmid#86969PurposeExpresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coliDepositorInsertPCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDHPromoterT7 promoterAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD2cX
Plasmid#86968PurposeExpresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coliDepositorInsertPCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…PromoterT7 promoterAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS-3
Plasmid#85094PurposeHost vector to fuse a gene at the N-terminus of PCNA3DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC3
Plasmid#85087PurposeExpresses PCNA3 protein fused to P450cam (CYP101A1) in E. coliDepositorInsertPCNA3 fused to P450cam
UseTagsHis6 tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa
Plasmid#46166PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cyprinodon variegatus cryaa
Plasmid#47664PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
CaMCLC-His
Plasmid#111292Purposebacterial expressionDepositorInsert(S)-malyl-CoA/b-methylmalyl-CoA/(S)-citramalyl- CoA lyase
UseTagsHis tag, GGGSGGGSLEHHHHHHExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
UseTags8 histidine tagExpressionBacterialMutationPromoterT7 promotorAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1
Plasmid#20061PurposeBacterial expression of membrane scaffold protein (MSP1D1) for Nanodisc formationDepositorInsertMSP1D1
UseTags7-His tagExpressionBacterialMutationdeletion mutant (1-11) of MSP1; His-tag on N-term…PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
Alpha-Synuclein-His
Plasmid#186802PurposeCodon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.DepositorInsertAlpha-Synuclein-His (SNCA Human)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR-spondin 1
Plasmid#128844Purposebacterial expression of pR-spondin 1DepositorInsertR-spondin 1 (RSPO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy49
Plasmid#55187PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and MBP tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…ExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
-
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-Nedd4L
Plasmid#186806PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.DepositorInsertHis-Tev-Nedd4L (NEDD4L Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.491
Plasmid#184956PurposeTest effect of a1/a2 length on editing rpoB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
UseTagsExpressionBacterialMutationrpoB donor T1534CPromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -