Addgene

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Showing: 4361 - 4380 of 10634 results

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pBAC-BA-lacZ

Plasmid

#13423

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

BA

Use

Cre/Lox

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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p6899 PHAGE-P CMVT N-HA-hE6AP II C843A

Plasmid

#37604

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II C843A (UBE3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C843A

Promoter

CMVt

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only
pMOD_A0000

Plasmid

#90997

Purpose

Module A, Promoter: none, Gene: none, Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTuLys2CMR2

Plasmid

#53544

Purpose

Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance

Depositor

Lingchong You

Article

Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

Insert

pLux

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

March 26, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Use

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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2 gRNA concatmer

Plasmid

#84879

Purpose

Empty concatmer vector in which 2 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only
pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

Plasmid

#137994

Purpose

Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

Depositor

Amy Brock

Article

Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJRA110

Plasmid

#209343

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal StayGold fusion protein.

Depositor

Lucia Strader

Article

Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pf hPGK mc2 IRES puro

Plasmid

#206237

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBJ1-rat Beta2m

Plasmid

#121670

Purpose

Expresses rat β-2-microglobulin in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat beta-2- microglobulin (B2m Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ICv-6MT

Plasmid

#41730

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Intracellular Domain (Notch1 Mouse)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein beginning at Vali…

Promoter

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pLKO-mito-mEm

Plasmid

#185596

Purpose

Lentiviral construct for expression of mEmerald targeted to mitochondria

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

mito-mEm

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pETduet+sfGFP-TetR

Plasmid

#157794

Purpose

Expression of sfGFP-TetR fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

sfGFP-TetR

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mSTIM1 myc

Plasmid

#17732

Depositor

Anjana Rao

Article

Oh-Hora et al Nat Immunol. 2008 Apr . 9(4):432-43.

Insert

STIM1 (Stim1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pECFP-Sec31A

Plasmid

#66612

Purpose

CFP fusion of Sec31A

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec31A (SEC31A Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pTRANS_150

Plasmid

#91205

Purpose

Transformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

PvUbi2

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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PB-Ostir-puro

Plasmid

#161972

Purpose

Expresses Ostir and puromycin resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-puroR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3001

Plasmid

#91042

Purpose

Module A, Promoter: 35S, Gene: GFP, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP

Use

Unspecified

Tags

Expression

Mutation

Promoter

35S

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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