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Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
MET-HYGRO
Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LYS-Leu2
Plasmid#218114PurposeIntegration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA
Plasmid#20298PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activationDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9Insertchannelrhodopsin-2
UseAAVTagsEYFPExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEC3135 (pERASE)
Plasmid#220306PurposeEnables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO3
Plasmid#24318DepositorInsertAGO3 (AGO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-ChETA-EYFP
Plasmid#26967Purpose3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInserthChR2(E123T/H134R)-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationE123TPromoterCaMKIIaAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64ExpressionMutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb-35S
Plasmid#161008PurposePP7-tagged reporter driven by the 35S promoterDepositorInsertPP7 and H2B-mScarlet
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
Plasmid#20297PurposeCre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorHas ServiceAAV Retrograde, AAV1, AAV5, AAV8, and AAV9Insertchannelrhodopsin-2
UseAAVTagsmCherryExpressionMammalianMutationhumanized ChR2 gene with histidine 134 changed to…PromoterEF1alphaAvailable sinceJune 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SH3BP1
Plasmid#136647PurposeExpression of SHBP1-GST fusion protein in E. coliDepositorInsertSH3BP1 (SH3BP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2
Plasmid#60625PurposeExpresses VP64 mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP64 mouse MyoD
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab5a
Plasmid#72901PurposeEarly endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab5a (RAB5A Human)
UseLentiviralTagsFKBP(F36V) and mCherryExpressionMammalianMutationfragment a.a. 2-215PromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO SwiChRca-TS-EYFP
Plasmid#55631PurposeCre-Dependent Chloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationC128APromoterEf1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4212
Plasmid#141313PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pComb3HTT
Plasmid#63900PurposeBackbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.DepositorInsertTT Fab
UsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CalfluxCTN
Plasmid#190068PurposeExpresses BRET Ca2+ sensor CalfluxCTN in mammalian cellsDepositorInsertCalfluxCTN
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only