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Plasmid#169421PurposeDonor DNA for TKIT knockin of SEP to GluA2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SARS2_XBB.1.5
Plasmid#196585Purposeexpressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus productionDepositorInsertSpike (XBB.1.5)
UseTagsExpressionMammalianMutationG252V, F486P on the basis of XBBPromoterCMVAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-E2-Crimson
Plasmid#197196PurposeExpresses the protein of E2-Crimson in mammalian cellsDepositorInsertE2-Crimson
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo
Plasmid#80766PurposeCustomizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorTypeEmpty backboneUseCRISPRTagsTagBFP2-3×NLSExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nme2Cas9_AAV
Plasmid#119924PurposeAll-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassetteDepositorInsertNme2Cas9
UseAAVTagsNLS-3xHA-NLS and NLS-NLSExpressionMammalianMutationPromoterU1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEa
Plasmid#193782PurposeExpress TadCBEa (with SpCas9) in mammalian cellsDepositorInsertTadA-CDa-SpCas9D10A
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2R
Plasmid#167932PurposelentiCRISPRv2 variant with mScarlet-I fluorescence marker.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPTN-EGFP
Plasmid#27052DepositorInsertoptineurin (OPTN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
PH-Akt(R25C)-GFP
Plasmid#51466PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationR25CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL1342
Plasmid#70691PurposeRSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCherry LIC cloning vector (6B)
Plasmid#30125PurposeEmpty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChrimsonR-tdT (AAV5)
Viral Prep#59171-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagstdTomatoAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-Traditional CAR (anti-CD19)
Plasmid#192115PurposeLentiviral delivery of Traditional CAR(anti-CD19)DepositorInsertpHR-pEF1a-Traditional CAR (anti-CD19scFV_CD28_41BB_CD3z)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer P-null replicon
Plasmid#65462Purposecontaining 1.3 units of the P-null HBV genome (subtype ayw)DepositorInsert1.3 units of the P-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST39-pNL29
Plasmid#91696PurposeExpresses Mega GVs in E.ColiDepositorInsertMega GV gene cluster (from pNL29) in pST39 backbone
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
JUP_pLX307
Plasmid#98347PurposeLentiviral expression of JUPDepositorInsertJUP (JUP Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBTK622
Plasmid#180592Purposetdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1DepositorInsertstdk
kanR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-FOSL2-P2A-mCherry
Plasmid#203860PurposeTet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertFOSL2 (FOSL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast
Plasmid#205550PurposeExpress EPN24-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN24-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only