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Plasmid#12275DepositorInsertH-Ras G12V E37G (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationContains G12V activating mutation. The E37G mutat…PromoterAvailable sinceJuly 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N35/3NQ
Plasmid#121455Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN192Q, N200Q and N219QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-mNeon-F-hgp100-PuroR [M1G]
Plasmid#171801PurposeUniversal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]DepositorInsertmNeonGreen-FLAG-hgp100-T2A-PuroR
UseGene taggingTagsExpressionMutationPuroR: Changed Methionin 1 to Glycine.PromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cenp-B-targeted Aurora B FRET sensor
Plasmid#45232DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA Oct4A -158
Plasmid#50922PurposeU6 driven sgRNA targeting OCT4 isoform A -158 bp from TSSDepositorInsertOct4A -158 sgRNA (POU5F1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro macroH2A1 shRNA
Plasmid#30517DepositorInsertmH2A1 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABE-2xFLAG-2xSTREP-CCND2-Puro
Plasmid#172628PurposeRetroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cellsDepositorInsertCCND2 (CCND2 Human)
UseRetroviralTags2xFLAG-2xSTREPExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W
Plasmid#163179PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorInsertYAP1 (YAP1 Human)
UseCRISPR and LentiviralTagsmCherryExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-MAPKAP1.1-FHH-IRES-Puro
Plasmid#120705PurposeExpresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 Isoform 1
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro-miR-10b sponge
Plasmid#25043DepositorInsertmiR-10b sponge
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA
Plasmid#162109PurposemCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; mCherry linked to StrepTagII-ProtC-HA
UseLentiviralTagsStrepTagII-ProtC-HAExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-CLIP-FLAG-CD59
Plasmid#50375PurposeExpress CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cellsDepositorInsertCLIP
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSUPER retro puro macroH2A2 shRNA
Plasmid#30518DepositorInsertmH2A2 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro/Flag PD-L1 N192Q/N200Q
Plasmid#121453Purposeexpression of human PD-L1 mutant in mammalian cellsDepositorInsertCD274 (CD274 Human)
UseLentiviralTagsFlagExpressionMammalianMutationN192Q/N219QPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 EGFP pBabe puro
Plasmid#13605DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseRetroviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc
Plasmid#161587Purposeexpression of Myc-tagged mouse Lrrk2 without GTPase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1023-1879PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p7270 MSCV-N-HA-IRES-PURO Empty
Plasmid#163302PurposeEmpty vector controlDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only