-
Plasmid#66868PurposeMammalian expression of SUMO2(Q87R) with a strep and an HA tagDepositorInsertSUMO2 (SUMO2 Human)
UseTagsHA and StrepExpressionMammalianMutationQ87RPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag MKK7a1 (K76A)
Plasmid#14541DepositorInsertMKK7a1(K76A) (Map2k7 Mouse)
UseTagsFlagExpressionMammalianMutationLys-76AlaPromoterAvailable sinceJuly 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9-HMGB1
Plasmid#183195PurposeExpression cloneDepositorInsertHMGB1
UseCRISPRTagsCas9WTExpressionMammalianMutationn/aPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA D665A pcDNA3
Plasmid#206113Purposeexpression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationD665APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A D491A HA pcDNA3
Plasmid#206109Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241A D491APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GFP-MTn-KDEL
Plasmid#154348PurposeMammalian expression of MTnDepositorInsertMTn
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-zeo-hFJX1-myc
Plasmid#46930Purposeexpression of human FJX1-myc in mammalian cellsDepositorInsertFJX1 (FJX1 Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
p5077 pcDNA4c hBrd4 (1-594)
Plasmid#14453DepositorInsertBrd4 (1-594) (BRD4 Human)
UseTagsHisExpressionMammalianMutationamino acids 1-594PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJA3
Plasmid#19493DepositorInsertDNAJA3 (DNAJA3 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-150
Plasmid#26310DepositorInserthsa-mir-150 (MIR150 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 L198A L232A
Plasmid#8978DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationL198A, L232APromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαq-124-NLuc
Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorInsertGNG2 (GNG2 Human)
UseTagsExpressionMammalianMutationN-terminally tagged with LSS-mKATE2PromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-MAVS-invitro(KaganD86)
Plasmid#52012PurposeUsed for in vitro expression of the human MAVS CDSDepositorInsertMAVS
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-PhoCl1-CBid
Plasmid#164050PurposeOptogenetic manipulation of cell apoptosis using PhoCl1DepositorInsertNBid-PhoCl1-CBid
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR(R89L)-NES
Plasmid#205568PurposeRasAR negative-control mutant plasmid. Targeted to cytosol.DepositorInsertRasAR(R89L)-NES (RAF1 Human, Synthetic)
UseTagsNuclear export signal (NES)ExpressionMammalianMutationArg 89 mutated to Leu in Raf1 RBD region.PromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-D614G
Plasmid#166850PurposeFor expression of SARS-CoV-2 Spike D614G in mammalian cells (C9 tagged at C-term)DepositorInsertSARS-CoV-2 Spike (S SARS-CoV-2 virus)
UseTagsC9ExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1+_FH-AGO2-5xA
Plasmid#92007PurposeExpresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]DepositorInsertAGO2 (AGO2 Human)
UseTagsFLAG-HAExpressionMammalianMutationS824A, S828A, T830A, S831A, S834A (5xA)PromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NBid-mMaple-CBid
Plasmid#164049PurposeOptogenetic manipulation of cell apoptosis negative controlDepositorInsertNBid-mMaple-CBid
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NGFP-VMAn-FKBP
Plasmid#70225PurposeExpresses N-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Glu 125 to Ile, Deleted amino acids 129-2…PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only