-
-
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCMV-intron myc Rab1aWT
Plasmid#46776Purposeexpression of WT Rab1a in mammalian cellsDepositorInsertRab1a (RAB1A Human)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO V5 HSPA1L
Plasmid#19511DepositorInsertheat shock 70kDa protein 1-like (HSPA1L Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_3xFLAG
Plasmid#101654PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, 3xFLAG tag
UseTags3xFLAG tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
UseTagsIntein-Chitin-binding domainExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- HIV2-ROD vpx
Plasmid#115816PurposeEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance proteinDepositorInsertHIV2ROD Vpx (M15390)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-SBP-STAG2 (WT)
Plasmid#73963PurposeSTAG2 cDNA (CCDS43990) wild typeDepositorInsertSTAG2 (STAG2 Human)
UseTagsFLAG-SBPExpressionMammalianMutationwild typePromoterAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB1
Plasmid#19495DepositorInsertDNAJB1 (DNAJB1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPML1 (GB0045)
Plasmid#68164PurposeProvides the Arabidopsis thaliana Meristem Layer 1 promoter as a level 0 GoldenBraid partDepositorInsertAtML1 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2p2A--3mnx1:mTagBFP-NS-MCS
Plasmid#78250PurposeCan be used to subclone in gene of interest into blue motor neuron expression vector.DepositorInsertsUseZebrafish motor neuron expressionTagsExpressionMutationPromoter-3mnx1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha CAT
Plasmid#16392DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationCAT: catalytic domain, S65DPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:YFP:Tnos (GB0209)
Plasmid#68219PurposeTranscriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoterDepositorInsertYFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
PKG I alpha dNPS
Plasmid#16391DepositorInsertPKG1 alpha (PRKG1 Human)
UseTagsHAExpressionMammalianMutationN-terminal pseudosubstrate domain deletion: delet…PromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-MCU
Plasmid#31726DepositorInsertMCU (MCU Human)
UsePdonr gatewayTagsNoneExpressionMutation*PromoterNoneAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9
Plasmid#84247PurposeExpresses direct fusion VPR-Sp dCas9DepositorInsertsVPR-tagBFP-Sp dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), HA tag, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos MAL Flag
Plasmid#41554DepositorInsertMAL (TIRAP Human)
UseTags6xHis and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9
Plasmid#135964PurposeExpresses SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only
