We narrowed to 4,711 results for: lum

1. pDawn

   Plasmid

   #43796

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is induced under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDusk

   Plasmid

   #43795

   Purpose

   Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

   Depositor

   Andreas Moeglich

   Article

   Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. Str-KDEL_neomycin

   Plasmid

   #65306

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
   

   Tags

   KDEL motif and Signal Sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEFIRES-P-mTagBFP-KDEL

   Plasmid

   #87163

   Purpose

   Fluorescent marker of endoplasmic reticulum (ER)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   mTagBFP-KDEL
   

   Tags

   Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

   Expression

   Mammalian

   Promoter

   EF-1 alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. iRFP-FRB-ER

   Plasmid

   #139317

   Purpose

   endoplasmic reticulum-resident FRB for chemically induced dimerization

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)
   

   Expression

   Mammalian

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmTurquoise2-ER

   Plasmid

   #36204

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   ER-mTurquoise2
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. LRTi

   Plasmid

   #214072

   Purpose

   Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

   Depositor

   Sujatha Jagannathan

   Article

   Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

   Insert

   Firefly luciferase
   

   Expression

   Mammalian

   Mutation

   Linker sequence present between firefly luciferas…

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRTX_ctrl

    Plasmid

    #214073

    Purpose

    Luminescent reporter system, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LRT_RTctrl

    Plasmid

    #214074

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LRT

    Plasmid

    #214071

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LiRT

    Plasmid

    #214075

    Purpose

    Luminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LiRT_RTctrl

    Plasmid

    #214078

    Purpose

    Luminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LiRTi

    Plasmid

    #214076

    Purpose

    Luminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Linker sequence present between firefly luciferas…

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LiRTX_ctrl

    Plasmid

    #214077

    Purpose

    Luminescent reporter system, NMD negative control. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    Firefly luciferase
    

    Expression

    Mammalian

    Mutation

    Remnant of linker sequence present at the end of …

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

    Plasmid

    #175530

    Purpose

    Expression of HaloTag7 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag7-KDEL
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMT-Bip-GFP:V5:KDEL

    Plasmid

    #69917

    Purpose

    An endoplasmic reticulum (ER) marker used to visulaize the ER structure in Drosophilia S2 cells.

    Depositor

    Kenneth Irvine

    Article

    Okajima et al Science. 2005 Mar 11;307(5715):1599-603.

    Insert

    GFP
    

    Tags

    Drosophila BiP signal sequence, KDEL ER targeting…

    Expression

    Insect

    Promoter

    pMT

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPV-DsRed2-ER-5

    Plasmid

    #170570

    Purpose

    Expresses DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after signal peptide cleavage.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    APV-DsRed2-ER-5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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