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Plasmid#107794Purposedonor vector for knock-in gene editing at human DNM1 locusDepositorInsertDNM1-eGFP (DNM1 Synthetic, Human)
UseYeast-e.coli shuttle vectorTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE CACNG8-GFP KI #1
Plasmid#131473PurposeEndogenous tagging of TarpÎł8: Intramolecular (amino acid position: V376)DepositorInsertgRNA and GFP donor (Cacng8 Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF
Plasmid#119864PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertBirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsHA-tagged biotin ligase (BirA), Thosea asigna vir…ExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR
Plasmid#126697Purposedonor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertTagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-YNL-CP
Plasmid#65714PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL-CP
Plasmid#65716PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL-CP
Plasmid#65715PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_BirA_CDKN1A
Plasmid#136523PurposeUsed as a donor vector to clone into pSLIKDepositorInsertCDKN1A (CDKN1A Human)
UseEntry vector for gateway cloningTagsExpressionMutationPromoterAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3-HPRT1-S104R-PdTK-mCh
Plasmid#107276PurposeHPRT1 c.312C>A Munich donor vector with unilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.306G>T, c.312C>APromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT
Plasmid#89890PurposeBAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.DepositorInsertHA-BirA-2A-citrine
UseUnspecifiedTagsCitrineExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pORANGE C9orf4-GFP KI
Plasmid#131472PurposeEndogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)DepositorInsertgRNA and GFP donor (Frrs1l Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRC2_p53DD
Plasmid#136520PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertp53DD (Trp53 Mouse)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationThis construct was created by PCR from the pBabe …PromoterAvailable sinceOct. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-hSOX10-Val15AlafsX11
Plasmid#24732DepositorInsert(sex determining region Y)-box 10 (SOX10 Human)
UseGateway donor vectorTagsExpressionMutationdeleted amino acids 44-62. deletion/frameshift…PromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
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p3-HPRT1-S104Rf-PdTK-mCh
Plasmid#107277PurposeHPRT1 c.312C>A (Munich) donor vector with bilateral microhomologyDepositorInsertsUseTagsExpressionMutationc.306G>T, c.312C>APromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
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