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Plasmid#25161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCYP51A1_cw_09 (CYP51A1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 54-502PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
p663-UBC-miniTurbo-V5-NEK9_IDG-K
Plasmid#189873PurposeGateway destination clone of NEK9 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNEK9 (NEK9 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRATIO1212
Plasmid#141299PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD363
Plasmid#91829PurposeLoxP-flanked SEC donor for the SapTrap cloning systemDepositorInsertLoxP-SEC-LoxP
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-NUAK2-V5-miniTurbo_IDG-K
Plasmid#189910PurposeGateway destination clone of NUAK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertNUAK2 (NUAK2 Human)
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECM1
Plasmid#75445PurposePlasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt1
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-01
Plasmid#125760PurposeEndlinker for tRNA-gRNA system when using only one guideDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-04
Plasmid#125763PurposeEndlinker for tRNA-gRNA system when using only four guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-05
Plasmid#125764PurposeEndlinker for tRNA-gRNA system when using only five guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH35
Plasmid#126009PurposeLevel0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-GDepositorInsertLevel0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-02
Plasmid#125761PurposeEndlinker for tRNA-gRNA system when using only two guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH55
Plasmid#125814PurposeLevel0 nSpCas9-PmCDA, Arabidopsis codon optimizedDepositorInsertnSpCas9-PmCDA, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
GGE083
Plasmid#120732PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_URA3)DepositorInsertMarker_URA3
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceOct. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNCX2
Plasmid#67148PurposeExpresses mRNA of NCX2 (Rat brain)DepositorInsertNCX2
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRegev_ExonSkip_LandingPad
Plasmid#204186PurposeBackbone for cloning individual exon sequences into beta globin minigene splicing reporter.DepositorInsertBeta globin splicing minigene
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTruncated CAGAvailable sinceAug. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF4C08
Plasmid#71059PurposeGateway entry cloneDepositorInsertmael (mael Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1B09
Plasmid#70833PurposeGateway entry cloneDepositorInsertrhi (rhi Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMZ283
Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRTagsExpressionYeastMutationPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH45
Plasmid#125810PurposeLevel0 nSpCas9-ABE7.10 base changer, human codon optimizedDepositorInsertnSpCas9-ABE7.10 base changer, human codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH34
Plasmid#126008PurposeLevel0 Arabidopsis AtU6-26 Pol III promoterDepositorInsertLevel0 Arabidopsis AtU6-26 Pol III promoter
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only