Showing: 4321 - 4340 of 4578 results
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Plasmid#171574PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membraneDepositorInsertBiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailabilityAcademic Institutions and Nonprofits only -
pAW-BiP-Nb127D01-mCherry-KDEL
Plasmid#171575PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membraneDepositorInsertBiP-Nb127D01-mCherry-KDEL (CXCR2 Human)
UseTagsBiP signal peptide and mCherry-KDELExpressionInsectMutationPromoterfly actin5C promoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
Plasmid#217511PurposeKD Recombinase dependent conditional smGdP-10XHA cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XHA
UseTagssmGdP-10XHAExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
Plasmid#217512PurposeKD Recombinase dependent conditional smGdP-10XOllas cassetteDepositorInsertKDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas
UseTagssmGdP-10XOllasExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRX-C751-E3616S
Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorInsertTRX (w Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
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K365-micro
Plasmid#188457PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-365 (Khc Fly)
UseTags6xHis-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-365PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIVTR(T7A)-Cas9n-RT-T2A-PuroR
Plasmid#172296PurposeIn vitro transcription of Cas9n-RT-T2A-PuroR synthetic construct for PRIME editingDepositorInsertCas9n-RT-T2A-PuroR
UseCRISPRTagsExpressionMutationH840A (Cas9)PromoterT7 promoter A-initiatingAvailabilityAcademic Institutions and Nonprofits only -
H3K9me3 biosensor
Plasmid#120802PurposeFRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRETDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
K9L mutant of H3K9me3 biosensor
Plasmid#120807PurposeFRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
UseTagsExpressionMammalianMutationlysine 9 is mutated to leucine 9 on histone H3PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
W45A mutant of H3K9me3 biosensor
Plasmid#120808PurposeFRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capabilityDepositorInsertTruncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
UseTagsExpressionMammalianMutationTryptophan 45 is mutated to Alanine 45 on HP1 dom…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83-GB1_AD
Plasmid#148514PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83DepositorInsertDmThor_50-63-DmeIF4Ga_631-638-DmThor_72-83 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmThor_50-63-DmeIF4Ga_631-675-GB1_AD
Plasmid#148515PurposeBacterial Expression of DmThor_50-63-DmeIF4Ga_631-675DepositorInsertDmThor_50-63-DmeIF4Ga_631-675 (Thor Fly)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeIF4E
Plasmid#79258PurposeExpresses EGFP-tagged DmeIF4E in S2 cellsDepositorInserteIF4E (eIF4E1 Fly)
UseSchneider 2 cell expressionTagsEGFPExpressionMutationPromoterAc5 promoterAvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUAS-Myc-BirA-G3-ER
Plasmid#173815PurposePromiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage siteDepositorInsertMyc-BirA-G3-ER
UseTagsMycExpressionInsectMutationPromoterUASAvailabilityAcademic Institutions and Nonprofits only -
Showing: 4321 - 4340 of 4578 results