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Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-mCherry
Plasmid#127887PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry taggingDepositorInsertPuro-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTmpGm
Plasmid#176229PurposeA template plasmid for amplification of the cI-hok-aacC4 cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, aacC4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-HBTH
Plasmid#127883PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HBTH taggingDepositorInsertGFP-Puro-HBTH
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-H3FAID
Plasmid#127884PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3FAID taggingDepositorInsertGFP-Puro-H3F-AID
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-HF
Plasmid#127882PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter H3F taggingDepositorInsertGFP-Puro-H3F
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTopo pLox hPGK-Puro pA pLox
Plasmid#171048PurposeEmpty donor template for CRISPR/Cas9 targeting of TERT RE'sDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA NuiHA-loxP-nat-CUP1p
Plasmid#131170PurposePCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to NourseothricinDepositorTypeEmpty backboneUseTagsNui-HAExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPD554 ZF129x6-Compact_EYFP
Plasmid#138868PurposeEYFP reporter vector for the ZF129x6-Compact promoterDepositorInsertZF129x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF129x6-CompactAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBC-DT1T2.2
Plasmid#134752PurposePCR template for sgRNA cloningDepositorInsertsgRNA-AtU6_29p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBC-MT1T2.2
Plasmid#134751PurposePCR template for sgRNA cloningDepositorInsertsgRNA-TaU3p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[1]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorInsertL-IFP-F[2]
UseVector for homologous recombinationTagsExpressionMutationPromoterTEF promoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD545 ZF37x6-Compact_EYFP
Plasmid#138859PurposeEYFP reporter vector for the ZF37x6-Compact promoterDepositorInsertZF37x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF37x6-CompactAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-EF1a-Puro
Plasmid#127170PurposePCR template for EF1a-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCfB8742
Plasmid#126908PurposeTemplate for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolyticaDepositorInsertpGPD1 and pFBA1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-BC-CMV-Puro
Plasmid#127169PurposePCR template for CMV-puro resistance cassetteDepositorInsertNone
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only