Addgene

We narrowed to 52,045 results for: cloning

Showing: 4301 - 4320 of 52045 results

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pGolden-AAV

Plasmid

#51424

Purpose

Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

lacZ

Use

AAV

Tags

Expression

Mutation

Promoter

lacZ

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-Kir2.1-mCherry-L2

Plasmid

#32669

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Kir2.1-mCherry

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 22, 2012

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pKER-mKOk

Plasmid

#85975

Purpose

Expresses mKOk from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mKOk

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL50008

Plasmid

#50314

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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Human NCKX6 (pcDNA3.1+)

Plasmid

#75216

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFGM1

Plasmid

#64949

Purpose

source of GmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-GmR-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NEK8_IDG-K

Plasmid

#189877

Purpose

Gateway destination clone of NEK8 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

NEK8 (NEK8 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA6

Plasmid

#19459

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA6 (HSPA6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pECT3

Plasmid

#75450

Purpose

Plasmid that serves as the PCR template to create genomic pdt3 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt3

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRATIO1112

Plasmid

#141066

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-ESYN-R5

Plasmid

#32581

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Enhanced Synapsin Promoter

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pRATIO2212

Plasmid

#141302

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCZGY2845

Plasmid

#87763

Purpose

Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pnmr-1-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pnmr-1

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18PV-mini-Tn7T

Plasmid

#64956

Purpose

pUC18-mini-Tn7T with unique PvuII site in pUC18 DNA

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCZGY546

Plasmid

#90466

Purpose

Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-mcherry

Use

Tags

mcherry

Expression

Worm

Mutation

Promoter

Pmec-4

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pBAC-ECFP-15xQUAS_TATA-SV40

Plasmid

#104875

Purpose

piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFH67

Plasmid

#131766

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 3):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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R26P-SA-EGFP-puro

Plasmid

#26890

Depositor

Rudolf Jaenisch

Article

Blelloch et al Proc Natl Acad Sci U S A. 2004 Sep 28. 1

Insert

EGFP

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pAM3110

Plasmid

#40247

Depositor

Susan Golden

Article

Ditty et al Microbiology. 2005 Aug;151(Pt 8):2605-13

Insert

attP Site

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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