Addgene

We narrowed to 20,167 results for: pET

Showing: 4281 - 4300 of 20167 results

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pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Use

Tags

His and thrombin cleavage

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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YPet-Golgi-7

Plasmid

#56633

Purpose

Localization: Golgi, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET32-sLatherin

Plasmid

#61685

Purpose

bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

Depositor

Brian Smith

Article

Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

Insert

Latherin (LATH horse)

Use

Tags

HIS and Trx-Tag thioredoxin protein

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

T7

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET21b-RL015A

Plasmid

#42134

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

sfGFP-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pFtnA-Ypet

Plasmid

#98280

Purpose

mammalian expression of FtnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Use

Tags

Antp

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET14b PHAS-I

Plasmid

#15679

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I (Eif4ebp1 Rat)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

Promoter

Available since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
pET23a-LIR-1 D1-D2

Plasmid

#12103

Depositor

Pamela Bjorkman

Article

Willcox et al Nat Immunol. 2003 Sep . 4(9):913-9.

Insert

Leukocyte immunoglobulin-like receptor 1 (LILRB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Amino acids 1−198*

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pET-UbcH10-wt

Plasmid

#8503

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pET28b-pchG WT

Plasmid

#87107

Purpose

expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

Insert

pchG WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
GSK3beta/pET29b

Plasmid

#16315

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

GSK3beta (gsk3b.L Frog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pET28-mTDGa.1

Plasmid

#81051

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Use

Tags

6HIS

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-NpR5600

Plasmid

#45741

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

NpR5600 (Npun_R5600 Nostoc punctiforme)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pET28b-irp3 WT

Plasmid

#87106

Purpose

expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

Insert

irp3 WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pETCON-DnvLB16

Plasmid

#45124

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

DnvLB16

Use

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Mutation

Promoter

GAL

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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