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Plasmid#61105PurposeExpression of Filamin with internal stFRET inserted to measure mechanical stress in real timeDepositorInsertfilamin-stFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMEXNH3
Plasmid#110079PurposeAnalogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Actinin-M-stFRET
Plasmid#61103PurposeExpression of α-actinin with internal stFRET inserted to measure mechanical stress in real timeDepositorInsertα-actinin-stFRET
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoVG-lexA
Plasmid#138405PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertV2 tail-TEVcs(ENLYFQG)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CA-FLEX-RG
Plasmid#38043DepositorInsertRabies virus glycoprotein
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAAvailable sinceAug. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorInsertOxysterol-binding Protein PH domain (OSBP Human)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas12c-sgRNA_BbsI_CAM
Plasmid#183221PurposeCas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate AssemblyDepositorInsertCas12c single guide RNA containing BbsI sites in spacer sequence
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-polySH33
Plasmid#175233PurposeBacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycinDepositorInsertNCK1 (NCK1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac GST-Plk4
Plasmid#80264Purposebaculovirus creation plasmid for GST-Plk4DepositorInsertPlk4 (PLK4 Human)
UseTagsGSTExpressionInsectMutationPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Arch-EEQ
Plasmid#45188PurposeArch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95NDepositorInsertenhanced Archaerhodopsin
UseLentiviralTagseYFPExpressionMammalianMutationD95Q, D106EPromoterCamKIIaAvailable sinceJuly 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationE59G - please see depositor commentsPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_W
Plasmid#173784PurposeThe Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.DepositorInsertWphi Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHelper_D
Plasmid#173781PurposeThe D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.DepositorInsertD145 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-TangoL-lexA
Plasmid#138404PurposeRMCE vector for generating C-terminal Tango fusionDepositorInsertTEVcs(ENLYFQL)-LexAp65
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB14
Plasmid#18657DepositorInsertUs9-EGFP gene fusion
UseTagsEGFPExpressionMammalianMutationFirst 106 amino acids of Us9 are intact. The stop…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
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p221z-AtMIR390a
Plasmid#118388PurposeEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertAtMIR390a backbone
UseRNAiTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-E2
Plasmid#175242PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylationDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mCER-Prx1
Plasmid#158227PurposeGenetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)DepositorInsertmCER-Prx1
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only