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P119 C-Check

Plasmid

#66817

Purpose

Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

Depositor

Yonglun Luo

Article

Zhou et al Cell Mol Life Sci. 2016 Jan 11.

Insert

truncated Enhanced Green Fluorescent Protein and asRED

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK and CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MAC-TMPRSS4

Plasmid

#172422

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

Transmembrane protease serine 4 (TMPRSS4 Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-EphB4-Fc

Plasmid

#200986

Purpose

Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB4

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-DICER-Prom

Plasmid

#25851

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

Dicer promoter (DICER1 Human, gene ID 23405)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

Plasmid

#74102

Purpose

plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Xenopus laevis beta-tubulin gene promoter region and 5'UTR
Green Lantern Green Fluorescent Protein
Rabbit beta 1-globin gene 3'UTR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1(A322D)-IRES2-EGFP

Plasmid

#170821

Purpose

human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

Depositor

Tingwei Mu

Article

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Insert

Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

change Alanine 322 to Aspartate

Promoter

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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FH1-PNGT#2-mCherry

Plasmid

#164099

Purpose

Fluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

PNGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-EphB1

Plasmid

#200981

Purpose

Mammalian expression plasmid for myc-tagged EphB1

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB1 (EPHB1 Human)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaAi9L-U6-SaAi9R

Plasmid

#78604

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

gRNAs for SaCas9 targeting Ai9 locus

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only
pTYF-PRSx8-AstR-p2A-GFP

Plasmid

#159632

Purpose

Lentiviral expression of the Allatostatin receptor, P2A GFP under the PRSx8 promoter

Depositor

Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

AstR (AstA-R1 Fly)

Use

Lentiviral

Tags

GFP and P2A

Expression

Mammalian

Mutation

Promoter

PRSx8

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-GP-2-KDM4A double Tudor

Plasmid

#59699

Purpose

Contains histone modification interacting domain (KDM4A double Tudor)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

KDM4A double Tudor (KDM4A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB3051(gRNA X-3 XI-2 XII-2)

Plasmid

#73293

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C

Plasmid

#171247

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor mutant that binds to bicalutamide: AR-Trp741Cys (AR-W741C)

Depositor

Steven Balk

Article

Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

Insert

3xFlag-tagged human androgen receptor Trp741Cys mutant (AR Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys (AR-W741C)

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry

Plasmid

#164097

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ACTB_sgRNA

Plasmid

#183884

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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